. BACTERIOLOGICAL TECHNIC. 79 



Echinulate, in agar stroke a growth along the line of inoculation, with toothed or 



pointed margins; in stab cultures growth beset with pointed outgrowths. 

 Effuse, growth thin, veily, unusually spreading. 

 Entire, smooth, having a margin destitute of teeth or notches. 

 Erose, border irregularly toothed. 



Filamentous, growth composed of long, irregularly placed or interwoven filaments. 

 Filiform, in stroke or stab cultures a uniform growth along line of inoculation. 

 Fimbriate, border fringed with slender processes, larger than filaments. 

 Floccose, growth composed of short curved chains, variously oriented. 

 Flocculent, said of fluids which contain pseudozooglea, i.e., small adherent masses of 



bacteria of various shapes and floating in the culture fluid. 



Fluorescent, having one color by transmitted light and another by reflected light. 

 Gram's Stain, a method of differential bleaching after gentian- violet, methyl- violet, etc. 



The + mark is to be given only when the bacteria are deep blue or remain blue after 



counterstaining with Bismarck brown. 

 Grumose, clotted. 



Infundibuliform, form of a funnel or inverted cone. 

 Iridescent, like mother-of-pearl. The effect of very thin films. 

 Lacerate, having the margin cut into irregular segments as if torn. 

 Lobate, border deeply undulate, producing lobes (see Undulate). 

 Long, many weeks or months. 



Maximum Temperature, temperature above which growth does not take place. 

 Medium, several weeks. 



Membranous, growth thin, coherent, like a membrane. 



Minimum Temperature, temperature below which growth does not take place. 

 Mycelioid, colonies having the radiately filamentous appearance of mould colonies. 

 Napiform, liquefaction with the form of a turnip. 

 Nitrogen Requirements, the necessary nitrogenous food. This is determined by adding to 



nitrogen-free media the nitrogen compound to be tested. 

 Opalescent, resembling the color of an opal. 



Optimum Temperature, temperature at which growth is most rapid. 

 Pellicle, in fluid bacterial growth either forming a continuous or an interrupted sheet 



over the fluid. 



Peptonized, said of curds dissolved by trypsin. 

 Persistent, many weeks, or months. 

 Pseudozooglece, clumps of bacteria, not dissolving readily in water, arising from imperfect 



separation, or more or less fusion of the components, but not having the degree of 



compactness and gelatinization seen in zooglea. 

 Pulvinate, in the form of a cushion, decidedly convex. 

 Punctiform, very minute colonies, at the limit of natural vision. 

 Rapid, developing in twenty-four to forty-eight hours. 

 Raised, growth thick, with abrupt or terraced edges. 

 Repand, wrinkled. 



Rhizoid, growth of an irregular branched or root-like character, as in B. mycoides. 

 Ring, same as Rim, growth at the upper margin of a liquid culture, adhering more or less 



closely to the glass. 



Saccate, liquefaction the shape of an elongated sac, tubular, cylindrical. 

 Scum, floating islands of bacteria, an interrupted pellicle or bacterial membrane. 

 Slow, requiring five or six days or more for development. 

 Short, applied to time, a few days, a week. 



