BACTERIOLOGICAL TECHNIC. 85 



2. Ammonia production, feeble, moderate, strong, absent, masked by acids. 



3. Nitrates in nitrate broth. 

 Reduced, not reduced. 



Presence of nitrites ammonia 



Presence of nitrates free nitrogen 



4. Indol production, feeble, moderate, strong. 



5. Toleration of Acids: Great, medium, slight. 

 Acids tested. 



6. Toleration of NaOH: Great, medium, slight. 



7. Optimum reaction for growth in bouillon, stated in terms of Fuller's scale 



8. Vitality on culture media: Brief, moderate, long. 



9. Temperature relations: 



Thermal death-point (10 minutes' exposure in nutrient broth when this is adapted 



to growth of organism) C. 



Optimum temperature for growth C.: or best growth at 15 C., 20 C., 25- 



C., 30 C., 37 C., 40 C., 50 C., 60 C. 



Maximum temperature for growth C 



Minimum temperature for growth C. 



10. Killed readily by drying: resistant to drying. 



11. Per cent, killed by freezing (salt and crushed ice or liquid air) 



12. Sunlight: Exposure on ice in thinly sown agar plates, one-half plate covered (times 15 



minutes), sensitive, not sensitive. 

 Per cent, killed 



13. Acids produced 



14. Alkalies produced 



15. Alcohols 



1 6. Ferments: Pepsin, trypsin, diastase, invertase, pectase, cytase, tyrosinase, oxidase, per- 



oxidase, lipase, catalase, glucase, galactase, lab, etc -. 



17. Crystals formed .... 



1 8. Effect of germicides: 



IV. PATHOGENICITY. 



1. Pathogenic to animals. 



Insects, crustaceans, fishes, reptiles, birds, mice, rats, guinea-pigs, rabbits, dogs, cats, 

 sheep, goats, cattle, horses, monkeys, man. 



2. Pathogenic to Plants: 



3. Toxins, soluble, endotoxins. 



4. Non-toxin forming. 



5. Immunity bactericidal. 



6. Immunity non-bactericidal. 



7. Loss of virulence on culture-media: Prompt, gradual, not observed in months. 



