THE MANUFACTURE AND USE OF SERA AND VACCINES. 141 



submit themselves for treatment. The earlier, after infection, the treatment 

 is begun the more likely will the results be satisfactory. The vaccine is, 

 however, now so prepared as to make home treatment possible: The 

 graded doses of the virus put up in sterilized ampuls are ready for imme- 

 diate use by the family physician. 



13. Streptococcus Mixed Vaccine. 



A mixed toxin of erysipelas (Streptococcus pyogenes and Bacillus pro- 

 digiosus} is used in the treatment of cancer and other malignant tumors, 

 particularly the sarcomas. It appears to have a local as well as systemic 

 effect. It is prepared as follows: Virulent cultures of the Streptococcus 

 pyogenes are grown in the incubator for three weeks, then inoculated with 

 the Bacillus prodigiosus and allowed to grow ten days longer at the room 

 temperature. The mixed cultures are then bottled, sterilized by heating 

 for an hour at 60 C., and are ready for use. Being unfiltered, the prepara- 

 tion is decidedly turbid in appearance. This preparation is administered 

 hypodermically, the injection being made in the neighborhood of the tumor 

 or into the tumor itself. 



14. Cancer Vaccine. 



Recently Dr. Gilman of Johns Hopkins University has experimented with 

 a cancer vaccine or cancer emulsion which it is believed will cure cancer. 

 It is simply a preparation made from the cancerous tissue of the patient, 

 macerated in a physiologic salt solution and preserved in some antiseptic. 

 This is suitably diluted and given in 10 c.c. doses injected directly into 

 the circulation. A reaction (rise in temperature to 102 to 104 F.), de- 

 velops in three days, suppuration ceases and the tissues begin to heal. The 

 dose is repeated three times at intervals of two weeks which is supposed 

 to be sufficient to effect a cure. Although not a bacterin as far as can 

 be ascertained, its action is evidently similar to that of an autogenous 

 bacterin. As yet it is too early in the history of the use of this remedy to 

 predict the final results. 



