540 APPENDIX. 



(47) Addition to 404, line 19. Some interesting experiments 

 on the influence of the injection of water into the blood simul- 

 taneously with blood-letting, have been made by Kierulf,* in 

 Ludwig^s laboratory. It would appear from these observations, 

 that a considerable attenuation of the blood generally gives rise to 

 a secretion of albumen through the kidneys, followed by hsematuria, 

 which, however, is probably not accompanied by laceration of the 

 capillaries of the kidneys. The rapidity with which the urine was 

 secreted bore no proportional relation to the amount of water con- 

 tained in the blood. 



(48) Addition to p. 406, line 1?. The fluctuations in the 

 amount of free acid in the urine during health have been made a 

 special object of attention by Bence Jones t and A. Winter. J 

 A. Winter found that an adult of average bodily weight (67 kilo- 

 grammes) discharged in the 24 hours as much free acid as would 

 correspond with 2*304 grammes of oxalic acid. (The amount of the 

 free acid was determined by means of a solution of ammonia 

 of known strength.) It appeared, moreover, from the experiments 

 of both these observers, that during the period of digestion, that is to 

 say, in the afternoon hours, the quantity of the free acid was at its 

 mean ; it attained its maximum during the night, and fell far 

 below the mean during the forenoon. It would appear from the 

 experiments of Jones, that the diminution of the free acid was more 

 decided after the use of animal food than after the use of mixed 

 food, and more especially of a vegetable diet, which is the more 

 remarkable, since we know that a purely vegetable diet gives a very 

 faint acid or even an alkaline reaction to the urine, whilst the latter 

 becomes very acid after the use of animal food (see my investi- 

 gations on the urine under different modes of diet), this being the 

 case, as Bernard has observed, even in herbivorous animals, which 

 usually discharge an alkaline urine. Nevertheless, these observa- 

 tions, which are in direct opposition to our own experiments, 

 deserve to be more carefully investigated. 



(49) Addition to p. 415, 14 lines from the bottom. The 

 ammonia of the ammoniacal salts passes for the most part un- 

 changed into the urine. 



* Mitth. der naturf. Ges. zu Zurich. Juli 1852. 

 f Philosophical Transactions. 1849, pp. 235-251. 

 :{: Op. cit. 



