1\! i'l'XC.I PARASITIC ri'O.N AI.K.Vkt >!>KS CITRI. 



it was desired to start the fungus. Further methods of introducing this 

 Asclvrsmiia by spraying the trees with water containing fungus spores, 

 obtained either from previously infected larvae or from artificial cultures, 

 have been recently carried on by E. W. Berger 2 ' 5 of the Florida Experi- 

 ment Station. Webber 24 had tried infecting larvae by spraying a mixture of 

 conidia in water, but had failed to reproduce the fungus in this way. E. W. 

 I Merger has found that to succeed with this method it is best to have a spray 

 pump that contains no copper parts, and that has also not been previously 

 used for spraying fungicides or insecticides. Fairly good infection of this 

 fungus has been obtained bv Merger, by the spore-spraying method, at St. 

 Petersburg, Leesburg. New Smyrna, Gainesville and Lake City. The fact 

 that infections may be made from cultures that have grown under artificial 

 conditions in the laboratory for long periods of time, suggests the possi- 

 bility of using these cultures in a practical way at the very beginning of 

 the rainy season, when fresh fungus on leaves is hard to obtain. Consid- 

 erable quantities of this fungus may be grown artificially on various media, 

 as will be shown in the following pages. 



C.Kk.M I. NATION OF CONIDIA. 



Conidia of this fungus were germinated in hanging drop cultures of dis- 

 tilled water, tap water, and various solutions of glucose. In all of these 

 cultures the germination was very slow, scarcely ever beginning in less 

 time than v?n hours. Germination in distilled water and tap water was very 

 feeble, while that in solutions of glucose was much stronger, as is explained 

 more fully under the germination tests for Aschcrsonia flaro-citrina. 



Trials were made at various times to germinate spores in hanging drop 

 cultures from pustules that had dried in the atmosphere of the laboratory. 

 The following is a record of these tests: 



/7 1. On November K), l!)()(i. cultures were made from 



// leaves collected on October IV. I'.HH;, which had remained 



/I in the laboratory vs days. The tests were made in glucose, 

 1 1 in distilled water, and in tap water. On December 11 (31 

 days) sporids formed in : per cent, glucose. The growths 

 Fit:.:;. "i distilled water and in tap water had not proceeded far 



" IIH SL - ( > January 5, 1907, cultures were made from leaves 



nonn 



collected October IV, lixxi, which had remained in the 



laboratory for s.~> days. The test was made in distilled water. \o germina- 

 tion took place. 



' Fl;i. Kxp. Sta. Bui. 88, ].p. 57-63, I'.MM;. 



"Webber, II. J. Div. of Veg. Phys, \- Path., Bui. 13, p. :>;. Washington, D. c. 



