1C Kr.\<,l PARASITIC rroN A I.KYROl >KS ClTRI. 



I. On I une 1!>, I'.HI;, bread medium was inoculated as the sweet potato 

 in No. :. with eonidia from a culture made on June :>, 1907. The old potato 

 plug from the same culture was also dropped in. ( )n July 1, a good growth 

 out from old plug had started. On July ><>. growth over nearly entire 

 surface, reddish in patches, had formed. On August 12, no eonidia could 

 l.e found. 



INI-KCTION 01- AI.KYKODKS CITKI 1-ROM cn.Tl'KKS. 



In two localities in the State, this fungus was started on larvae of 

 .-llcvrodcs citri, from cultures which had grown for some time on culture 

 media in the laboratory. This infection work was done by E. W. Berger 

 at Gainesville and St. Petersburg, from cultures grown by the author. 

 The following records are taken from E. \Y. Merger's field notes: 



1. . \ugtist 1(). HOT. Gainesville. From culture on sweet potato made June :>:\. 

 having been isolated not later than April 10, 1907. Culture mixed up in water and 

 sprayed with hand sprayer on under surface of leaves on lower branches of an orange 

 tree. October 6, no fungus found. On Xovemher 1(>. three pustules of fungus found. 

 On December :M. sprinkling of pustules evident. April ::. 1!H)S. quite a sprinkling of 

 fungus pustules evident. 



:.'. August 10. I'.ioT. Gainesville, Florida. From old cultures in three test-tubes. 

 Age of cultures unknown. No start by April :;. I'.ios. 



::. August 1.">, I'.ioT. St. Petersburg, Florida. From culture made June !'., on 

 bread in large bottles and transferred twice before from cultures isolated not later 

 than April 10. The culture was washed to fine pulp in water and strained. One and 

 a half to two quarts of the liquid was used. Sprayed on the under side of leaves with 

 a compressed-air sprayer. On October :'!!, quite a sprinkling of fungus was found. 

 Some twigs had several leaves well covered with pustules, mainly on the newest 

 growth. On December ](). about the same condition. On February 8, 190S, an abund- 

 ance of fungus was found on northwest side of tree, and fresh pustules were appearing. 



t. August 1". I'.iOT. St. Petersburg, Florida. From culture on sweet potato, 

 made June 1'.i. I'.iOT. Culture washed to fine pulp and strained. Two quarts of solu- 

 tion were sprayed on with compressed-air hand sprayer. On October :Z3. a good 

 sprinkling of fungus was found with some leaves well covered. P>est catdi was on 

 we>t side, mainly on newer growth. On December 10, I'.IOT. pustules more mature. 

 but apparently not spreading. On February s I'.ios. an abundant catch of fungus was 

 evident. 



5, August i:,. I'.MiT. St. Petersburg. Florida. From a mixture of two cultures, 

 one of Ascfarsonta alcyrndis made June I'.i on potato plug and transferred twice 

 before; the other of . Isi'licrsaiihi flai'o-citrina made May ::;. mi sweet potatoes in a 

 bottle, and transferred twice before. Cultures mixed, washed and strained, making 

 one gallon of the solution. On October '.':;, fair sprinkling of the fungus, probably 

 only the red. mainly on newer growth. ( )n December 10. same, but pustules more 

 matured On February 8, I'.-os. all red pustules, no yellow pre-uit. 



<; August 14. I'.iOT. St. Petersburg. Florida. From culture made June I'.i. I'.iOT. 

 and transferred twi.-e before. \o start by February 8, 1908. 



\ugust M. I'.iOT. St. Petersburg. Florida. From culture made April s. I'.HMi, 

 which had been dried for two months. The fungus bad been transferred twice' before. 

 Isolated from petri dish culture made December T. I'.ioi;. \o start of fungus by Feb- 

 ruarv .s. itMis. 



