368 PROTEIN-COMPOUNDS. 



they are immersed, and thus render it denser ; in which case the 

 blood-corpuscles again contract, become denser and flatter, and 

 are again visible. 



No properties have yet been detected in coagulated globulin 

 by which it may be distinguished from other boiled protein- 

 compounds. 



Composition. Globulin has been subjected to even fewer 

 analyses than vitellin ; as that which is contained in the blood can 

 never be perfectly freed from haematin, no accurate analysis can be 

 made of it. Dumas* has however analysed a specimen containing 

 hsematin, while both Mulderf and Ruling have analysed this sub- 

 stance as obtained from the crystalline lens. 



Ruling. 

 54-2 



1-2 

 lOO'O lOO'O 



Although Berzelius assumed that phosphorus as well as 

 sulphur was contained in this substance, Mulder found only the 

 latter, which averaged O265$ : this sulphur was however determined 

 in the moist way ; in the dry way, I determine the sulphur in 

 globulin from the crystalline lens of the calf (as a mean of three 

 experiments) at 1*134?, and RiilingJ in globulin similarly obtained 

 from the ox at 1*227?-. Mulder, at present, regards globulin as a 

 combination of his hypothetical protein with sulphamide. 



The globulin of the crystalline lens contains only a very small 

 amount of insoluble ash-constituents; I found only 0*241$ of 

 phosphate of lime. 



In globulin from the crystalline lens of a calf I found 1*548$ of 

 soluble salts consisting of metallic chlorides, sulphate of soda 

 ( = 30-37? of the soluble salts) and alkaline phosphates ( = 7'77? of 

 the soluble salts), but containing no alkaline carbonates. On the 

 other hand, on evaporating the fluid filtered from the coagulated 

 globulin (which besides 92*095$ of coagulated globulin yielded 

 7*905$ of soluble residue) I obtained on the incineration of this 

 residue an ash which contained only 13*166$ of phosphate of lime, 



* Compt. rend. T. 22, p. 904. 



t Journ. f. pr. Ch. Bd. 19, S. 189 ; and Bullet, d. Ne'er!. 1839, p. 196. 



Ann. d. Ch. u. Pharm. Bd. 58,'S. 313. 



