650 METABOLISM 



Some of the urea may come from metabolic processes of an entirely 

 different type. One of these at least is known ; namely, the splitting-off 

 of urea from arginine, which it will be remembered is guanidine-amino- 

 valerianic acid (see page 640). An enzyme called arginase, having this 

 action, has been isolated from various organs and tissues. The diamino- 

 valerianic acid, or ornithine, which remains after the urea is split off, 

 may be further used in protein metabolism. The reaction is shown in 

 the following equation: 



NH 2 - C - NH - CH 2 - CH 2 - CH 2 - CHNH 2 - COOH + H 2 O 

 NH (arginine) 



NH 2 -CO 



I 4- NH 2 - CH 2 - CH 2 - CH 2 - CHNH 2 - COOH 

 NH 2 

 (urea) (ornithine) 



On an ordinary diet, as we have seen, a man excretes somewhat more 

 than 90 per cent of his total nitrogen as urea and about 3 per cent as 

 ammonia, the remainder of the nitrogen appearing in the other nitrog- 

 enous metabolites. 



Influence of Acidosis on Ammonia-Urea Ratio. It sometimes happens 

 that a large proportion of the ammonia is used for the purpose of neu- 

 tralizing abnormal acids present in the organism. The ammonia is appar- 

 ently produced in the kidney (page 563). When mineral acids are given 

 to an animal, or when acids are produced in the organism itself by some 

 faulty type of metabolism, the ammonia excretion by the urine immedi- 

 ately rises. In diabetes, for example, where considerable quantities of 

 /?-oxybutyric acid are produced (see page 715), a decided increase in the 

 ammonia excretion is observed. A milder type of acidosis may also be in- 

 duced in normal persons by withholding carbohydrates from the diet, and 

 here again the ammonia excretion is relatively increased. 



In such cases the ammonia is to be regarded as an alkaline re- 

 serve in the sense that it carries the excess of acids out of the body 

 and so saves fixed alkalies. It does not appear, however, that all 

 types of acidosis entail the utilization of ammonia as reserve alkali, 

 and an increase in the relative amount of ammonia in the urine does 

 not necessarily indicate a condition of acidosis. In the pernicious 

 vomiting of pregnancy, for example, a relatively high excretion of am- 

 monia has been found associated with no greater a degree of acidosis 

 than in normal cases of pregnancy, as determined by the power of the 

 plasma to absorb carbonic acid. When there is a relative excess of al- 

 kali in the blood (alkalosis) the ammonia excretion becomes depressed, 

 as is the case after taking alkali with the food, or in the alkalosis pro- 

 duced by forced breathing (page 382). 



