EXCRETORY FUNCTION OF THE LIVER. 281 



for cholesterine were not quantitative. In the succeeding 

 experiments, the proportion of cholesterine in the different 

 specimens of blood was accurately estimated, and, in most 

 of them, no anaesthetic was used during the operative pro- 

 cedure. 



Experiment II. A medium-sized adult dog was put un- 

 der the influence of ether, and the carotid artery, internal 

 jugular, and femoral vein exposed. Specimens of blood 

 were drawn, first from the internal jugular, next from the 

 carotid, and last from the femoral vein. These specimens 

 were received into carefully -weighed vessels, and weighed. 



They were then analyzed for cholesterine by the process 

 already described, and the following results obtained : 



Quantity of Blood. Cholesterine. Cholesterine per 

 grains. grains. 1,000 pte. 



Carotid 179-462 0'139 0'774 



Internal jugular 134'780 O'lOS O'SOl 



Femoral vein 133-886 0-108 0'806 



Percentage of increase in the blood from the jugular over the arterial 



blood 3-488 



Percentage of increase in the blood from the femoral vein 4'134 



This experiment shows an increase in the quantity of 

 cholesterine in the blood in its passage through the brain, 

 and an increase, even a little greater, in the blood passing 

 through the vessels of the posterior extremity. To facilitate 

 the operation, however, the animal was brought completely 

 under the influence of ether, which, from its action on the 

 brain, would not improbably produce some temporary dis- 

 turbance in the nutrition of that organ, and consequently 

 interfere with the experiment. For the purpose of avoiding 

 this difficulty, we performed the following experiments with- 

 out administering an anaesthetic : 



Experiment III. A small young dog was secured to the 

 operating-table, and the internal jugular and carotid ex- 

 posed on the right side. Blood was taken, first from the 

 jugular, and afterward from the carotid. The femoral vein 



