PRODUCTION OF. SUGAR IN THE LIVER. 311 



that passed through was tested for sugar by Trommer's test. 

 The reaction was doubtful, and presented no marked evidence 

 of sugar. 



Experiment II. A medium-sized dog, in the same con- 

 dition as the animal in the first experiment, was held upon 

 the table, and a portion of the liver excised, as above de- 

 scribed. The whole operation occupied twenty-two seconds. 

 But ten seconds elapsed from the time the portion 'of the 

 liver was cut off until it was in the boiling water. It was 

 boiled for about fifteen minutes, made into a paste with 

 animal charcoal, and thrown upon a filter. The clear fluid 

 that passed through was tested for sugar by Trommer's test. 

 There was no marked evidence of sugar. 



Experiment III. A large dog, full grown, and fed regu- 

 larly every day, but not in digestion at the time of the 

 experiment, was held firmly upon the table. This dog had 

 been in the laboratory about a week, and was in a perfectly 

 normal condition. The abdominal cavity was opened, and 

 a piece of the liver cut off and thrown into boiling water, 

 the time occupied in the process being ten seconds. Be- 

 fore the liver was cut up into the boiling water, the blood 

 was rinsed off in cold water. The liver was boiled for about 

 seventeen minutes, mixed with animal charcoal, and the 

 whole thrown upon a filter. 



Immediately after cutting off a portion of the liver and 

 throwing it into boiling water, the medulla oblongata was 

 broken up ; a ligature was applied to the ascending vena 

 cava, just above the renal veins ; the chest was opened, and 

 a ligature applied to the vena cava, just above the opening 

 of the hepatic veins. A specimen of blood was then taken 

 from the hepatic veins. This portion of the operation occu- 

 pied not more than one minute. A little water was added 

 to the blood, which was boiled briskly, mixed with animal 

 charcoal, and thrown upon a filter. The liquid that passed 

 through from both specimens was perfectly clear. 



While the filtration was going on, Fehling's test-liquid 



