GONOCOCCUS AND PNEUMOCOCCUS VACCINE. 77 



time determined upon by the opsonic index. The doses injected 

 in these cases varied from five hundred million to one thousand 

 million cocci. 



Treatment of acute and chronic urethritis by vaccines has 

 given some, but relatively few, beneficial results, and is by no 

 means to be regarded as a sufficient measure to obtain a cure from 

 this condition. 



Micrococci pneumoniae Infections. Apparently some good re- 

 sults have been obtained in the treatment of chronic infections 

 with this organism. The dose injected varies from ten million to 

 one hundred million. In acute pneumonia the course of the 

 disease is usually too short to derive beneficial results by means 

 of active immunization. 



Tuberculosis. The bacillus of tuberculosis may produce its 

 lesions in almost any part of the body. Bacteriological examina- 

 tion of the lesions produced will vary. In some lesions, especially 

 those localized in the interior of the body such as the glands, 

 joints and bones, the tubercle bacillus alone is responsible for the 

 condition found. In other lesions, as in pulmonary and intestinal 

 tuberculosis, there are present in addition to tubercle bacilli other 

 bacterial species. Active immunization, by the injection of killed 

 tubercle bacilli theroetically will be of benefit in lesions due to 

 the tubercle bacillus alone, while in lesions produced by tubercle 

 bacilli and other species it will not be very efficacious. 



In the specific treatment of tuberculous conditions, various 

 modifications of so-called tuberculin have been used. Old tuber- 

 culin is the concentrated germ-free glycerine bouillon on which 

 tubercle bacilli have been grown for several weeks. Before fil- 

 tration the tubercle bacilli are killed by heat. Deny's bouillon 

 filtrate, "B. F." differ from old tuberculin in that filtration is done 

 before killing the bacteria by heat. Tuberculin R, "T. R. "con- 

 tains the powdered tubercle bacilli suspended in salt solution. 

 Usually 1 c. c. contains 2 m. g. of powdered bacilli. Tuberculin 

 0, "T. 0." is the supernatent opalescent liquid obtained after 

 emulsifications of ground up tubercle bacilli in distilled water, 

 are centrifuged. Bacillen Emulsion, "B. E." is a suspension of 

 ground up tubercle bacilli in fifty per cent glycerine. It contains 

 5 m. g. in 1 c. c. of suspension. 



