TUBERCULINE, COLON AND TYPHOID BACILLUS VACCINE. 79 



guides in tuberculin treatment of tuberculosis. Wright injects 

 only amounts large enough to produce a positive phase in the 

 opsonic index and in some cases of tuberculous glands and joints 

 has accomplished good results by autoinoculation. Trudeau, 

 Denys and others have regulated the dosage and inter-spacing of 

 injections by clinical signs and symptoms. They usually inject 

 1-1000 m. g. of old or 1-10,000 m. g. of "Bacillen Emulsion." 

 After this, by a long continued treatment they gradually increase 

 the dosage. The doses injected are not increased nor the interval 

 between injections shortened if there are any general or local re- 

 actions, as evidenced by fever, local redness and edema. In this 

 method no stress is laid on the absolute amount of tuberculin 

 injected. When the patient has no tolerance to tuberculin, Tru- 

 dean has usually observed chronic toxaemia and progression of 

 the disease. 



Tuberculin treatment has proven especially beneficial in 

 lesions in the joints, bones, glands, skin and urinary bladder, 

 although good results have been obtained in pulmonary and other 

 forms of tuberculosis. 



Bacillus coli Infections. Different infections produced by 

 Bacillus coli have been treated by injections of vaccines made with 

 this organism. The vaccine method of treatment has been found 

 to be most successful in cases of chronic cystitis due to B. coli com- 

 munis, although cholecystitis, appendix abscesses, endometritis 

 and other local infections with this organism, have at times re- 

 sponded favorably to this method of treatment. The number of 

 bacteria injected is seldom more than fifty million, usually smaller 

 doses than this being used. Vaccines made from cultures isolated 

 from the patient usually give the best results. The injections are 

 repeated about every ten days. 



Bacillus typhosus and Bacillus paratyphosus Infections. The 

 typhoid bacillus group consist of several important members. 

 Recognition of this fact has led to the use of vaccine made from 

 cultures isolated from the patient to be treated. Vaccines made 

 from killed cultures of typhoid bacilli have been used for immuniz- 

 ation as well as for treatment of pathological conditions due to 

 members of this group. 



Pfeifler and Kolle have made vaccines for immunization 

 against typhoid fever from agar cultures, while in England the 



