the method of preparation of the fatty acids need not be uniform but 

 may be optional with the analyst. 



(3) Variations of the methods of stirring were found to give very 

 different figures. The Wolfbauer scheme of constant stirring until 

 the thermometer stopped falling gave uniformly higher results, as 

 was shown last year. In addition to the data given in Tables I and II, 

 the following results obtained by M. H. Ittner are of value: 



TABLE III. Titer tests by M. H. Ittner, using special method of stirring and different 



methods of preparation. 



The figures given are averages of three determinations and the 

 results show that these three methods of preparing the fatty acids do 

 not affect the titer. Others reported the same experience. 



W. D. Richardson reports the following results, which indicate that 

 after the fatty acids are dried by filtering very little effect is caused by 

 drying at 105 C. The Wolfbauer method was used: 



Titer, C. 



Not washed, filtered, nor dried 43. 



Washed, not filtered nor dried 42. 9 



Washed, filtered, not dried .,..43.2 



Washed, filtered, dried at 105 C. for 10 minutes 43. 2 



Washed, filtered, dried at 105 C. for 20 minutes 43. 4 



Washed, filtered, dried at 105 C. for 30 minutes 43.4 



All these results agree with those obtained by Wolfbauer, who con- 

 cluded that the method of saponification did not affect the titer, provid- 

 ing that the alcohol is completely removed in alcoholic saponification. 

 A. V. H. Mory, however, calls attention to the fact that there is greater 

 danger of scorching the fat by the Wolfbauer method of saponification, 

 but finds that if the soaps are carried down to dryness over an asbestos 

 sheet this contingency can be avoided. The scorching of the fat 

 reduced the titer to a considerable extent, as is shown in the follow- 

 ing table: 



TABLE IV. Effect on titer of scorching the soaps, by A. V. H. Mory. 



