PROTOCOLS OF EXPERIMENTS 



75 



From these lice the following preparations were made: 



Louse No. 



/Smears 



W 261 

 W 262 



W 263 

 W 264 



^Sections 



W 258 

 W 259 

 W 260 



Result of Microscopical 

 Examination 



Rickettsia absent 

 Rickettsia absent 



Rickettsia absent 

 Rickettsia absent 



Rickettsia absent 

 Rickettsia absent 

 Rickettsia absent 



Result of Animal Inoculation 



Guinea-pig 25. Died in five days 



Guinea-pig 26; Temp. + 



Histology negative 



Guinea-pig 27; Temp. 



Guinea-pig 28; Temp. - 



Proved susceptible to blood from a 



typhus patient 



Record of Guinea-pig 25, inoculated with the viscera of 

 rickettsia-free louse W 261 



This guinea-pig died on the fifth day, of pneumonia. 



Record of Guinea-pig 26, inoculated with the viscera of 

 rickettsia-free louse W 262 



The temperatures following the inoculation on May 16th 

 were as follows: 



May 16 101.8 May 27 102.0 



17 102.4 



18 102.6 



" 19 102.4 



" 20 102.1 



21 102.0 



22 102.2 



" 23 102.4 



" 24 No record 



25 101.9 



26 101.3 



June 5th; killed. Cultures from the heart's blood in dextrose 

 broth remained sterile (fifty-six hours). The autopsy was nega- 

 tive except for an enlarged spleen. 



Microscopic examination was negative for all tissues, includ- 

 ing the brain. 



