PROTOCOLS OF EXPERIMENTS 

 From these lice the following preparations were made: 



Result of Microscopical 



Examination Result of Animal Inoculation 



Rickettsia absent 

 Rickettsia absent 



81 



Louse No. 



Smears 



W265 



W266 



W267 



Rickettsia absent 



Guinea-pig 29; Temp. 

 Doubtful immunity test 

 Guinea-pig 30; Temp. 

 Proved susceptible to blood from a 

 typhus patient 

 Guinea-pig 31 ; Temp. 



Record of Guinea-pig 29, inoculated with rickettsia-free 

 louse W 265 



The temperatures following inoculation on May 16th were 

 as follows: 



May 16 101.4 



17 . . 103.9 



June 



18 

 19 

 20 

 21 

 22 

 23 

 24 

 25 

 26 

 27 

 28 

 29 

 30 

 31 

 1 

 2 

 3 



103.0 

 103.2 

 103.4 

 102.8 

 102.2 

 102.8 

 102.8 

 103.0 

 103.2 

 103.4 

 102.6 

 101.4 

 102.0 

 102.2 

 103.0 

 103.0 

 102.2 



. 



June 4 102.4 



5 103.0 



6 103.2 



7 102.8 



8 102.4 



9 101.8 



10 102.2 



11 102.6 



12 102.0 



13 101.8 



14 102.0 



15 102.0 



16 No record 



17 No record 



18 102.0 



19 102.4 



20 No record 



21 . . 102.4 



On June 22d this guinea-pig was inoculated intraperitoneally 

 with 5 c.c. of blood from patient 799 (eighth day of typhus). 

 The temperatures were as follows: 



June 22 No record 



23 104.2 



24 No record 



25 103.6 



26 No record 



27 102.8 



28 102.5 



29 No record 



30 . . 102.7 



July 1 102.5 



2 No record 



3 104.2 



4 103.6 



5 103.8 



6 No record 



7 102.6 



8 102.7 



9 . .No record 



