PROTOCOLS OF EXPERIMENTS 101 



Record of Guinea-pig 43, inoculated with the viscera of 

 louse 3 of Box XXXVII 



Temperatures following the inoculation on May 24th: 



May 24 102.5 June 11 101.8 



25 102.4 12 . . 101.2 



26 102.4 



27 102.6 



28 102.8 



29 103.0 



30 102.9 



13 101.8 



14 102.0 



15 101.8 



16 No record 



17 No record 



31 102.0 18 102.5 



June 1 101.8 19 102.0 



2 102.0 20 No record 



3 101.9 21 102.6 



4 101.8 22 No record 



5 102.6 " 23 102.3 



6 102.2 24 No record 



7 102.4 25 102.4 



8 101.8 26 No record 



9 102.0 27 102.0 



10 101.8 



On June 28th this guinea-pig was inoculated intraperitone- 

 ally with 5 c.c. of blood from patient 814 (eighth day of ty- 

 phus). The subsequent temperatures were as follows: 



F. F. 



June 28 102.5 July 8 102.8 



29 No record 



" 30 102.4 



July 1 102.8 



" 2 No record 



3 103.8 



4 103.2 



" 5 103.1 



6 No record 



7 . . 102.9 



9 No record 



10 103.4 



11 102.8 



12 103.1 



13 104.9 



14 105.0 



15 No record 



16 104.8 



17 . . 104.2 



July 17th; killed. The autopsy was negative except for an 

 enlarged spleen covered with a thin transparent layer of 

 fibrin-like material. 



Result: Guinea-pig 43, inoculated with the viscera of louse 3 

 of Box xxxvn, during an observation period of thirty-four 

 days did not develop typhus. Fifteen days after inoculation 

 with human typhus blood it developed, coincidently with 

 others receiving the same blood, the reaction of typhus. 



Conclusion: Louse 3 of Box xxxvn did not contain the 

 virus of typhus. 



