PROTOCOLS OF EXPERIMENTS 



105 



human typhus blood on June 28th, it developed typhus, coin- 

 cident with other guinea-pigs inoculated with the same blood. 

 Conclusion: Rickettsia-free louse 1 of Box xxxvm did not 

 contain the virus of typhus. 



Record of Guinea-pig 46, inoculated with the viscera of 

 rickettsia-free louse 2 of Box XXXVIII 



Temperatures following the inoculation on May 24th : 



June 



2 102.2 



3 . . 102.2 



103.0 

 102.8 

 105.2 

 104.0 

 104.3 

 104.2 



June 9th; killed. The autopsy showed a spleen of twice 

 the normal size, without exudate. All other tissues were nega- 

 tive. Histological examination showed the characteristic 

 lesions of typhus in the brain. 



Two guinea-pigs, Nos. 74 and 75, were inoculated, each 

 intraperitoneally, with 3 c.c. of blood from the heart of this 

 guinea-pig 46. 



The temperatures of these two guinea-pigs follow: 



No. 74 



No. 75 



F. 



No record 



103.8 



103.0 



104.2 



104.4 



103.0 



102.0 



No record 



No record 



102.8 



102.8 



No record 



102.8 



No record 



102.2 



102.8 



No. 74 



