CONTROL CULTURES 115 



cultures from this case. From Case 165 a short chained strep- 

 tococcus developed in the flask of glucose broth. 



Results: Under presumably favorable conditions, we failed 

 in fourteen attempts to cultivate from typhus patients a 

 bacillus resembling that described by Plotz. 



ADDITIONAL AEKOBIC BLOOD CULTURES 



Blood cultures were taken on eight cases, to determine the 

 frequency of cases with which terminal invasions of the blood 

 stream occurred. The cultures were taken on the following 

 cases: Nos. 96, 87, 83, 106, 109, 113, 157 (blood cultures were 

 made in this case on two successive days). These were all 

 cases in critical condition and, in the majority of instances, 

 moribund. 



The media used were glucose broth, glucose agar, and plain 

 agar. In a few of the cases, bile media was used in addition. 



Results: All the cultures were negative except that from 

 No. 157. The first culture in this case showed three distinct 

 types of organisms present: a large gram-positive bacillus; 

 a very small gram-negative bacillus; and a gram-positive 

 coccus that proved to be staphylococcus albus. The culture 

 from this case was repeated and the same types of organisms 

 were recovered again. 



