The plant material remaining in the Soxhlet apparatus was allowed 

 to dry and about 200 cc of ethylic ether placed in the flask of the appa- 

 ratus. The ethereal extract was colored green and about ten days were 

 required for complete extraction. On treating the residue obtained from 

 the ethereal extract by evaporation with water, the aqueous solution 

 was acid to phenolphthalein and contained a resin which was found to 

 be soluble in alcohol, partially soluble in ether, and which reduced 

 Fehling's solution. This residue was carefully tested for all the sub- 

 stances enumerated by Dragendorff as likely to appear at this point, but 

 no indication of anything other than resin was obtained. On account 

 of the small amount of the residue (0.2675 gram=1.33 per cent) its 

 physiological action was not tested. That portion of the residue of the 

 ethereal extract not soluble in water was tested physiologically by mix- 

 ing about one-half gram of the material with meat and feeding it to a 

 cat. The animal experienced no noticeable inconvenience from this 

 large dose, and it is to be concluded that the medicinal virtues of the 

 plant do not reside in the water-insoluble ether extract. 



The alcoholic extraction of the plant material was effected in a stop- 

 pered Erlenmeyer flask, using 200 cc of absolute alcohol and the sample 

 remaining after the ether extraction. The flask was shaken by hand at 

 intervals during five weeks, after which time the solution was filtered 

 through a dry filter and the plant material washed sufficiently with 

 absolute alcohol to remove the total alcoholic extracted portion. The 

 washings were received in a separate vessel. The tannin was deter- 

 mined by treating the residue obtained from an 'aliquot portion of the 

 alcoholic extract with water and adding lead subacetate to the filtered 

 aqueous solution. The precipitate obtained was washed, dried, and 

 weighed, after which the organic material was incinerated and the lead 

 remaining weighed, the loss being stated as tannin and extractives. 

 The water-insoluble portion of the residue obtained from the alcoholic 

 extract was a black, amorphous mass corresponding to 0.86 per cent of 

 the plant. This residue was completely soluble in dilute ammonia and 

 appeared to be a portion of the same resin which the ether had failed 

 to remove in the previous extraction of the plant. Although the material 

 extracted by alcohol appeared to be principally tannin, it was thought 

 desirable to test its action on a cat. The dose administered, however, 

 as was to be expected, produced no evident unusual physiological action. 



The water extract of the sample was made in the same flask which 

 had been used for the alcoholic extraction. Two hundred cc of water 

 and the dried plant material remaining after the alcoholic extraction 

 were used. At the end of twenty-four hours the aqueous solution was 

 filtered through a dry filter, the plant material washed several times 

 with water and the washings discarded. An aliquot portion of the 

 filtrate was titrated with standard alkali. The total extracted material 

 and ash were determined in another portion of the filtrate. The muci- 



