12 



CAFFEIN IN NEPHRECTOMIZED RABBITS. 



be exceptional, as inspection of the protocols of the next two series 

 shows that much greater amounts of caffein were eliminated also by 

 this channel. 



SERIES II AND III. Nephrectomized rabbits which were chloroformed 22 hours after 

 subcutaneous injection of caffein. Diet, oats. 



The increased rate of elimination of caffein by the intestines was 

 also shown in Series II and III. In both series of experiments 

 elimination was more marked by the stomach than was found to be 

 the case in Series I, or in normal rabbits, the amount of caffein re- 

 covered in the gastric contents being 3.5 and 3.3 per cent of the 

 amounts administered, which is fully twice the quantity recovered 

 in Series I, or in rabbits with kidneys intact. The elimination of 

 caffein by the intestinal epithelium was considerably less than in 

 Series I, but was more marked than in the normal rabbits just referred 

 to. In the next series of experiments the rate of elimination was 

 found to be appreciably greater in the stomach and was also much 

 more marked in the intestines. 



ELIMINATION OF CAFFEIN IN FROM 7 TO 28 HOURS (SERIES IV). 



Rabbit 738. Gray; weight, 1,725 grams. 



February 1: 10 a. m., double nephrectomy; 3.20 p. m., 17 cc of 2 per cent caffein 

 injected subcutaneously; 4.30 p. m., hypersensitive; 10 p. m., found dead. 



Rabbit 789. Gray; weight, 1,643 grams. 



February 2: 10.30 a. m., double nephrectomy; 3.20p.m., 16.5 cc of 2 per cent caffein 

 injected subcutaneously. 



February 3: 2.30 p. m., chloroformed, contents of stomach and intestine removed 

 and examined for caffein. 



Rabbit 741. Gray; weight, 1, 685 grams. 



February 2: 3.30 p. m., double nephrectomy; 3.45 p. m., 17 cc of 2 per cent caffein 

 injected subcutaneously. 



February 3: 4 p. m., chloroformed and contents of stomach and intestine removed. 



Half an hour after receiving caffein all the rabbits of this series 

 showed symptoms of caffein intoxication, such as increased reflexes, 

 photophobia, and restlessness. Of these three subjects rabbit No. 738 

 was found dead 12 hours after the nephrectomy and 7 hours after 

 the injection of caffein. Rabbit No. 738 was run independently 

 for a determination of the caffein recovered. 



