ELIMINATION OF CAFFEIN IN NEPHRKCTOMIZED BABBITS. 17 



considerable bleeding; 1.30 p. m., received subcutaneously 150 mg caffein per kilo; 

 2.30 p. m., strongly hypersensitive, restless, and avoiding the light; 5 p. m., died 

 in tetanic convulsions; caffein found in intestinal tract, 4.32 per cent; in stomach, 5 

 per cent; total caffein recovered, 10.77 per cent; duration of life three and one-half 

 hours; 15 cc urine; no caffein. 



Rabbit 835. In late pregnancy; weight, 2,220 grams. 



July 18, 1912: 11.45, laparotomized, as rabbit No. 834, except the bile duct was not 

 ligated; 1.30 p. m., received subcutaneously 150 mg caffein per kilo; 2.15 p. m., 

 hypersensitive and restless; 3.45 p. m. , clonic convulsion; died; duration of life two 

 and one-fourth hours; caffein in intestines, 4.32 per cent; in stomach, 3.1 per cent; 

 uterus and contents, 8.1 per cent; total caffein, 15.72 per cent; no urine secreted, for 

 none found in bladder. 



Normal rabbits. July 18, 1912: Caffein elimination, four hours after subcutaneous 

 injection of 150 mg caffein per kilo. 



Caffein recovered from normal rabbits. 



In Series IX elimination into the gastrointestinal canal varied 

 between 12.1 and 14.9 per cent, which is higher than in the case of 

 nephrectomized rabbits. It will be noticed, however, that with one 

 exception the amount recovered from the urine was insignificant, 

 while in one case no caffein at all could be detected. It is significant 

 that in rabbit No. 839, in the gastrointestinal canal of which the 

 smallest amount was found, the quantity of caffein in the urine was 

 higher than hi the other rabbits, thus pointing to a decided tendency 

 to vicarious elimination. The experiments with rabbits Nos. 834 

 and 835 apparently contradict this statement, but the vastly increased 

 toxicity of caffein in these two cases is probably responsible for the 

 decreased elimination of caffein into the gastrointestinal canal. 

 Additional experiments were therefore undertaken to ascertain the 

 relation between elimination in the urine and into the gastrointestinal 

 canal. 



ELIMINATION OP CAFFEIN IN RABBITS IN WHICH URINE WAS ABUNDANT (SERIES X). 



Caffein was given to a number of rabbits. Only those were used 

 in which diuresis was marked. 



Rabbit 861. Pregnant; diet, oats and cabbage; water consumed, 100 cc. 



September 7, 1912: 11.30 a. m., received 17 cc of 2 per cent caffein injected subcu- 

 taneously, or 150 mg per kilo; 3.50 p. m., bladder emptied; total urine, 150 cc. Per 

 cent caffein recovered in urine, 6.41; stomach, 2.93; intestines, 5.42; total, 14.76. 



Rabbit 864. Diet, oata and cabbage; water consumed, 100 cc. 



