Beginning in September I96O, two samples were taken at each 

 station at third-points and composited in a half -gal 1 on bottle. 



All of the samtples were sent to the Department of Public Health, 

 Sanitation and Radiation Laboratory, Berkeley, for identification and 

 enumeration. The results of this study axe reported in Appendix C. 



Attached Plants . Attached plants were collected at several 

 locations by pulling the entire plant loose from the subtrate and preserved 

 in approximately five percent formsLLin solution. 



Bottom Organisms . Bottom organisms were collected by means 

 of a Surber sampler or dredge. Collections were made with a one square- 

 foot Surber sampler at miles 297-7, 29k, 285-9, 279.2, 275, 256.3, 253. U, 

 2I+I.0, and 229.8. Three samples were taken on most occasions at these 

 stations. The entire contents in the sampler, which included organisms, 

 detritus, sand, smftll gravel, etc., were labeled and preserved in 10 per- 

 cent formalin. 



A Petersen dredge was used to collect bottom organisms at all 

 of the stations other than those listed above, from May i960, through 

 the end of the sampling program. Station depths ranged from k to 30 feet. 

 The area sampled by this dredge was 96 square inches, or approximately 

 two-thirds squeire foot. From one to three dredge hauls were m ade for 

 each collection. Attempts at using an Ekman dredge were unsuccessful. 



Samples collected with the dredge were poured into soil sieves 

 with openings of O.589 mm (Standard U. S. Sieve No. 30) . The organisms 

 remaining on the screen were picked off with forceps, placed in vials, 

 together with appropriate labels, and preserved in a 10 percent formalin 

 solution. 



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