Laboratory Procedures 



Bottom Orgemisms 



Bottom organism samples were delivered to the Brjrte Laboratory 

 of the Department of Water Resources for analysis. Samples of bottom 

 organisms were placed in Petri dishes and examined under a binocular dis- 

 secting microscope. 



The animals were separated into the lowest most easily recog- 

 nizable taxonomic group, and placed by group in Syracuse watch glasses 

 for more refined determinations. Each group was then more closely examined 

 and identified to the lowest possible taxonomic unit (e.g. genus and/or 

 species) . After identification, the numbers and volume of each kind of 

 organism were tabxilated. The sample material was then placed in a vial, 

 together with a collection data slip, and preserved in alcohol for per- 

 msuient storage. The laboratory form, storage vial, and collection data 

 slip all have the same inscribed number so that cross references can be 

 made. These samples are stored by the Department of Fish and Game at 

 the Sacramento Field Station, and are available for future reference. 



Two measurements were used in the q.u6uatitative analysis of the 

 bottom fauna sauiples. These included numbers and volume of individueils . 

 The volume of displacement was calciilated by placing the portion of the 

 sample to be measured on absorbent paper toweling and permitting the indi- 

 viduals to diy for one -half to one minute. A meas\ired amount of distilled 

 water was pipetted into a gradxiated cylinder. The blotted animals were 

 then admitted. The volinne of the organisms was recorded in cubic centimeters. 



Bottom Sediment Size 



Particle size analyses of bottom sediment samples were made 

 in accordance with the procedure given in the Department of Water Resources' 



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