12 DETERMINATION OF SUGARS IN GRAIN AND CATTLE FOODS. 



extraction are important. The latter point is generally taken into 

 account by chemists, but temperature is not always considered 

 unless special note is made of it. Directions to conduct extractions 

 at room temperature are rather vague, for this temperature during 

 the summer varies from that during the winter; and, again, some 

 chemists prefer to work at a much lower temperature during the win- 

 ter than others, and this plays an important part in the process. 

 However, in the sodium carbonate method, the time, two and one-half 

 hours, with occasional shaking, has been found long enough to remove 

 the sugars under nearly all temperature conditions. 



When using the greatest of care with the sodium carbonate method 

 the results of determinations made at different times on the same 

 sample seem to show a fair degree of agreement, as shown by the 

 following results: 



Duplicate analyses made at different times by the sodium carbonate method. 



The results by the alcohol method show possibly a little better 

 agreement, as given in the following table: 



Duplicate analyses made at different times by the alcohol method. 



