PART VI. 
APPENDIX. 
CHAPTER XXL 
LABORATORY WORK. 
Laboratory work should, so far as possible, accompany the study 
of the text. It is impossible to make a series of experiments that will 
closely follow the order of topics in the text, since a large amount of pre- 
liminary work has to be done in making media before actual study of 
bacteria begins. The order of experiments below given will be found a con- 
venient one to follow, but may be modified to suit convenience. 
APPARATUS NEEDED. 
Steam sterilizer. 
Autoclave. 
Hot air sterilizer A common gas oven used for cooking will do. 
Stew-pan for cooking media. 
Flasks. Liter and half-liter. 
Test-tubes, heavy Board of Health pattern. 
Petri dishes four inches in diameter. 
Pipettes i c.c. and 2 c.c. Some larger ones are also convenient. 
Each of the smaller ones should have a glass tube holder (Fig. 56) or there 
should be a metal box with cover to hold fifty or more pipettes at once. 
Wire baskets to hold test-tubes. 
Test-tube racks, or common tumblers with a little cotton in the bottom 
to hold test-tubes. 
Beakers. 
Evaporating dishes. 
Fermentation tubes. 
Burette holder with four burettes. 
Evaporating dishes. 
Measuring cylinders i liter and 100 c.c. 
Counting plate or counting card. 
Platinum wire to be fused into glass rods. 
Culture oven with constant temperature of 98. 
Bunsen burners. 
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