STUDY OF BACTERIAL CULTURES 



111 



Blood-serum. This is inoculated in the same manner as the 

 agar slope, and the same characteristics are to be noted, with the 

 addition of liquefaction or digestion of the medium. 



Gelatin Stab. This is prepared by running an inoculated plat- 

 inum needle in a straight line from the surface of an erect tube 

 of nutrient gelatin nearly to the bottom, and withdrawing it with- 

 out cutting the medium by any lateral motion of the needle. 



Fig. 58. Potato slant culture (Page, Frothingham and Paige, in 

 of Medical Research"). 



Journal 



The characters to be noted are abundance and uniformity of 

 growth along the line of the stab, the form of growth, liquefaction, 

 and other changes in the medium. 



Nutrient Broth. This is inoculated by shaking an infected 

 platinum needle in the medium. The characters to be noted are 

 abundance and character of surface growth and character of 

 sediment. 



Milk. Milk is inoculated in the same manner as the nutrient 



