406 



VETERINARY BACTERIOLOGY 



Isolation and Culture. The organism may readily be isolated 

 from pus from the lesions. Potato is the most favorable medium. 

 Original isolations show at the end of a week, transplants at the 

 end of two or three days, as white, filamentous colonies. These 

 enlarge, become darker at the center, and finally turn dark 

 brown or black, frequently surrounded by a rim of white. The 



colony becomes wrinkled. Upon gela- 

 tin the growth remains white. Lique- 

 faction begins in from three to ten 

 days or even later. The addition of 

 dextrose causes the center of the col- 

 onies to darken. In agar, and partic- 

 ularly in neutralized glycerin agar, 

 growth is good, and the colonies 

 remain white. Blood-serum is not 

 liquefied. In litmus milk growth 

 occurs with little change in the 

 medium, or coagulation without acid 

 production may take place after the 



jjjhjff' lapse of several weeks. In liquid 



J media growth occurs in the form of 



4 more or less separated colonies, usually 



accompanied by a surface pellicle. 



Physiology. The organism is an 

 obligate ae'robe. Growth occurs best 

 at 25 to 28, but is not prevented 



Fig. ISl.-Sporotrichum at 37 ' The spores resist desicra- 

 beurmauni, cultures and col- tion for considerable periods. Acid 

 onies on potato (Page, Froth- j s produced from dextrose, but not 



tzk, " d M& R : f I-*-. mait -. ~* h - 



search"). n ^ e > dulcit, adonit, inulin, or raf- 



finose. Gas is not produced from 



any sugar. No indol is formed. Gelatin is slowly liquefied. 

 The organism is destroyed at a temperature of 60 for five 

 minutes. 



Pathogenesis. Experimental Evidence. There is an abundance 

 of evidence that Sporotrichwn beurmanni is pathogenic for man and 

 animals. Accidental laboratory infections have taken place in 



