

492 VETERINARY BACTERIOLOGY 



animal inoculation and by macroscopic examination. For the 

 former it is customary to inject an emulsion from the brain into a 

 rabbit. The inoculation is usually made subdurally. Sections 

 or smears may be made from the brain and stained to show the 

 characteristic Negri bodies. Small portions of the gray substance 

 are removed from the cerebral cortex in the region of the crucial 

 sulcus, the cerebellar cortex, and the hippocampus major. These 

 are crushed on a slide and a smear made by means of a cover-glass. 

 These dried smears may be stained by Giemsa or other stains, 



Fig. 214. Method of drying the spinal cord of a rabbit for the purpose of 

 attenuation (Stimson, Bull. No. 65, Hygienic Laboratory). 



perhaps most readily by the method described by Williams and 

 Lowden: "To 10 c.c. of distilled water three drops of a saturated 

 alcoholic solution of basic fuchsin and 2 c.c. of Loffler's solution of 

 iiH-thylcnc-Mur arc added. The smears are fixed while moist in 

 methyl alcohol for one minute. The stain is then poured on, 

 warmed till it steams, poured off, and the smear is rinsed in water 

 and allowed to dry." 



Transmission. The saliva of diseased animals is found to be 

 infective, and the disease is transmitted commonly through the bite. 



