DEPARTMENT OF AGRICULTURE. 



A second series of experiments was performed on cattle. The first group of these 

 was as follows : 



The second group of observations is indicated below : 



Fifteen days after the inoculation small tumors were observed at the root of the tail, 

 causing this organ to be slightly raised. In one the tumor speedily disappeared; in 

 the other the swelling enlarged, became very hard, attained the size of a hen s egg, was 

 situated between the anus and the root of the tail, and yielded gradually, without suppu 

 rating, to scarifications and a saline purgative. 



Of the five other bullocks four showed no signs; the fifth, three weeks after the 

 inoculation, manifested a swelling of the head on the operated side. Two incisions were 

 made, emollients applied, and a purgative administered. Low diet was also prescribed. 

 On the 20th of April the whole side of the head was swollen and almost of scirrhous hard 

 ness. Two deep incisions were made without finding pus. In the nose, at the point where 

 the inoculation was performed, was a wound of unhealthy aspect from which a sanious pus 

 was discharged. The ox grew lean. On the 17th of May a little pus flowed from the two 

 incisions made on the 20th of April; afterward much pus flowed from these incisions, as 

 well as shreds of areolar tissue and portions of dead skin. The tumor was subsiding. On 

 the 22d of May a fluctuating tumor appeared below the jaws, from which much indolent- 

 looking pus escaped. From that moment the ox began to thrive, notwithstanding that the 

 suppurations continued till the 5th of June. By the 10th of June recovery was complete. 

 Dr. Willems despaired for several days of this animal s return to health, and he resolved 

 not to inoculate again in the same region. 



THIRD GROUP OF OBSERVATIONS. 



On the 10th of May Dr. Willems inoculated nine Dutch bullocks and two lean Belgian 

 cows. He made two punctures in the tail of each, and used blood expressed from the 

 muscles and liquid squeezed out of the lung of a cow suffering under the third stage of 

 pleuropneumonia. 



Several bullocks showed the effects of inoculation by the 19th of May; two more 

 severely than the rest. On the 21st of May there was a decided swelling of the tail iu 



