EXPERIMENTS WITH CBYPTOGAMIC GROWTHS. 161 



Experiment .5, February 25, 1869. One of the vacuum tubes of blood from the 

 above-mentioned cow, and a tube of healthy blood which had been put up at the same 

 time, were opened and carefully examined. The blood in each was coagulated, free from 

 offensive odor, and under the microscope presented no unusual appearance. The contents 

 of each tube were placed in a one-ounce vial with a slip of purified blotting paper, the 

 vials sealed and kept at a temperature of 70 Fahrenheit. Ten days later bacteria and 

 vibriones were present in each, but no trace of mycelium or of fungus fructification. 



On the 26th of February, 1869, a cow in the last stages of pleuropneumonia was 

 killed near Washington, and vacuum tubes were filled from the jugular vein. Tubes were 

 also filled with the serum contained in bulloa formed by the false membrane lining the 

 bronchial tubes. 



About four inches of each jugular vein were removed, ligatures having been first 

 applied. Eighteen hours afterward the blood in the veins from which the tubes had been 

 filled was carefully examined with a power of 750 diameters. It was coagulated, and the 

 serum contained some molecules, single or in chains of two or three, which were motion 

 less, (see plate, fig. 1.) Blood from one of the vacuum tubes contained no such bodies. 

 The lung serum contained molecules like those in the vein. 



Experiment 6, February 26, 1869. In a culture apparatus were placed three watch- 

 glasses and two growing slides, arranged as follows: The growing slides and watch-glass 

 No. 1 contained boiled potato and diseased blood; watch-glass No. 2 contained boiled 

 potato and healthy blood ; watch-glass No. 3 contained boiled potato and lung fluid. Twenty- 

 four hours later, in the growing slides the red corpuscles had nearly disappeared; bacteria 

 and monads, single or in short chains, were seen; a few moving, but the greater part at 

 rest. Seven days later there was no change ; motionless bacteria and monads were present 

 in all the glasses, but no trace of mycelium or spores. 



Experiment 7, February 26, 1869. Seven watch-glasses and five growing slides 

 were arranged as follows: AVatch-glass No. 1 contained potato boiled in distilled water; 

 watch-glass No. 2 contained lemon boiled in distilled water; watch-glass No. 3 contained 

 lemon boiled with diseased blood; watch-glass No. 4 contained diseased blood alone; watch- 

 glass No. 5 contained healthy blood alone ; watch-glass No. 6 contained boiled potato with 

 diseased blood; watch-glass No. 7 contained boiled potato with healthy blood; growing 

 slide A contained boiled lemon with diseased blood; growing slide B contained boiled 

 lemon with healthy blood; growing slide contained boiled potato with diseased blood; 

 growing slide D contained boiled potato with healthy blood; growing slide E contained 

 boiled potato alone. These were placed in four sets of culture apparatus, and kept at a 

 temperature of 78 Fahrenheit. In twenty-four hours a few small cells were seen in slide 

 B, which rapidly developed into ordinary yeast, continuing to bud and increase for four 

 days. . The fluids in watch-glasses 4 and 5 rapidly putrefied, and were filled with bacteria 

 and monads. In watch-glasses 1 and 2 and growing slide E no change had occurred in 

 eight days. In the others a few motionless bacteria appeared on the second day, after 

 which there was no change. The precautions taken in this experiment to exclude extra 

 neous bodies were great, embracing every point which could be thought of as liable to lead 

 to error. In April one of the tubes containing lung serum from this cow was given to Mr. 

 Reid, residing near Washington, and with its contents he successfully inoculated several 

 cattle, producing in each case the same effects, and, judging by the after results, conferring 

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