PREPARATION OF ANTI-HOG-CHOLERA SERUM 105 



the blood of each hyper is stored separately so 

 that if the animal should show on autopsy general- 

 ized tuberculosis or other disqualifying disease, 

 the blood may be discarded. Local conditions 

 must govern these details. In our own laboratory 

 where it is possible to purchase hypers which 

 have not followed cattle and have not been fed raw 

 creamery products, we always mix bleedings, in 

 spite of the fact that we do not clarify the serum. 

 We have never had a hyper show generalized 

 tuberculosis, and in the few instances in which 

 we have found the disease localized, we have used 

 a guinea-pig test to determine the fitness of the 

 serum for market. We believe this test has a 

 wider application than is now accorded it. 



FINAL PROCESSES IN SERUM PRODUCTION 



Testing. Irrespective of detail in preparation, 

 the final requirement is that the finished product 

 shall pass a test proving its protective potency 

 under carefully controlled conditions. For this 

 purpose there is drawn a sample from a mixed lot 

 consisting usually of about 100,000 mils of serum. 

 Seven pigs weighing between 45 and 90 pounds 

 each are selected for the official Bureau of Animal 

 Industry test. Temperatures must be normal and 

 all pigs in good physical condition at the time the 

 test begins. Each pig is given 2 mils of hog 

 cholera virus, five of them receiving in addition, 



