262 HOG CHOLEEA 



following paragraph, shall be thoroughly sterilized by 

 live steam at a temperature of at least 120 C. for not 

 less than one-half hour, or, by dry heat at a temperature 

 of at least 160 C. for not less than one hour. If for any 

 reason such methods of sterilization are impracticable, 

 then a process known to be equally efficacious in de- 

 stroying microorganisms and their spores may be sub- 

 stituted after approval by the chief of bureau. 



Paragraph 2. Instruments used in connection with 

 the bleeding of virus pigs and hyperimmune hogs, and 

 other like equipment, of establishments manufacturing 

 hog-cholera virus and anti-hog-cholera serum, which are 

 found to be damaged by exposure to the degree of heat 

 prescribed in the preceding paragraph, after having been 

 thoroughly cleaned, may be sterilized by boiling for not 

 less than 15 minutes, provided apparatus, satisfactory 

 to the inspector in charge, is furnished for this purpose. 



REGULATION 10. STORAGE 



SECTION 1. Viruses, serums, toxins, and analogous 

 products which may be injuriously affected by exposure 

 to light or to high temperature shall be stored in a dark, 

 cold chamber or refrigerator at a temperature of not to 

 exceed 55 F. All dealers in the District of Columbia 

 or any Territory or in any place under the jurisdiction 

 of the United States shall keep such products protected 

 from light and under refrigeration until sold or other- 

 wise disposed of. 



REGULATION 11. RECORDS 



SECTION 1. Paragraph 1. Permanent detailed rec- 

 ords of the sources, of the preparation, of tests for purity 

 and potency, and of methods of preservation, of each 

 batch of virus, serum, toxin, and analogous products 

 shall be kept by each licensed establishment and by each 



