276 HOG CHOLERA 



Paragraph 10. Hog-cholera virus shall not be re- 

 moved from the premises of a licensed establishment un- 

 less the virus has been prepared in accordance with the 

 provisions of these regulations. 



Paragraph 11. No immediate or true container of 

 hog-cholera virus shall be filled in whole or in part, and 

 no trade label shall be affixed to such containers except 

 under the supervision of a bureau employee. 



Paragraph 12. The following special facilities, and 

 such others as may be required by the chief of bureau, 

 shall be provided by each establishment licensed to pre- 

 pare hog-cholera virus: 



(a) Separate operating rooms. 



(6) A separate room in which the animals shall be 

 washed, cleaned, and otherwise prepared before being- 

 taken into the operating room. 



(c) A separate room for conducting autopsies. 



(d) A separate room for the preparation and mixing 

 of virus. 



(e) A separate room for washing and sterilizing 

 equipment. 



(/) Clean cloths which shall be kept damp when in 

 use, to be used for covering pigs during all operations 

 incident to the collection of hog-cholera virus. 



(g) All outside screens, openings, and windows shall 

 be equipped with dust screens. 



Paragraph 13. All persons, immediately before en- 

 tering the operating or laboratory rooms of an establish- 

 ment licensed to prepare hog-cholera virus, shall change 

 their outer clothing or cover it by the use of clean 

 gowns, or other satisfactory garments. 



SECTION 2. Paragraph 1. For use in the production 

 of hyperimmunizing virus, licensed establishments shall 

 inoculate young pigs weighing not more than 145 pounds 

 each with at least 2 cubic centimeters of a virulent strain 

 of hog-cholera virus. 



