APPENDIX 277 



Paragraph 2. Hyperimmunizing virus prepared in 

 accordance with sections 1 and 2 of this regulation may 

 be transported from one licensed establishment to an- 

 other under bureau seal, provided the product is prop- 

 erly iced and its transfer is accomplished in such man- 

 ner and by such methods as shall be approved by the 

 chief of bureau. 



SECTION 3. Paragraph 1. For use in the production 

 of simultaneous virus, licensed establishments shall in- 

 oculate young pigs weighing not less than 40 pounds, 

 nor more than 100 pounds each with at least two 

 cubic centimeters of a virulent strain of hog-cholera 

 virus. 



Paragraph 2. Simultaneous virus shall not be col- 

 lected from pigs which become visibly sick on or before 

 the fourth day, or subsequent to the seventh day after 

 the time of inoculation. The physical condition of pigs 

 from which simultaneous virus is collected shall be re- 

 corded daily on and after the fourth day subsequent to 

 inoculation. 



Paragraph 3. Simultaneous virus shall be collected 

 only from pigs which exhibit visible symptoms of hog 

 cholera within seven days, and are visibly sick of this 

 disease to a degree sufficient to result in death within 

 15 days after the time of inoculation. 



Paragraph 4. Pigs which have been inoculated for 

 the production of simultaneous virus shall be killed only 

 after permission has been obtained from an authorized 

 bureau employee. 



Paragraph 5. Simultaneous virus shall be defibri- 

 nated promptly after collection, and immediately there- 

 after chilled and maintained at a temperature not to 

 exceed 55 F. (12.8 C.). 



Paragraph 6. When simultaneous virus is heated it 

 shall be done under the supervision of a bureau employee 

 and in such manner as to subject the product and the 



