278 HOG CHOLERA 



entire container thereof to a temperature ranging from 

 50 to 50.5 C. for 12 hours. 



Paragraph 7. Simultaneous virus which has been 

 heated, as provided in the preceding paragraphs, shall 

 not be handled thereafter in a manner which will ex- 

 pose the product to contamination. 



Paragraph 8. When simultaneous virus is heated, as 

 described in paragraph 6 of this section, and tested upon 

 pigs, as hereinafter provided, the product need not be 

 tested upon calves. 



Paragraph 9. When simultaneous virus is heated, each 

 batch shall be tested for virulence by inoculating intra- 

 muscularly, with two cubic centimeters of virus, each 

 of two pigs which are susceptible to hog cholera. Should 

 the pigs thus inoculated exhibit visible symptoms of hog 

 cholera, as required for pigs inoculated to furnish simul- 

 taneous virus, the virus under test may be marketed. 



Paragraph 10. Pigs selected for testing the virulence 

 of heated simultaneous virus shall be inoculated imme- 

 diately after their admission to the premises. The quar- 

 ters where these pigs are held during the test shall be 

 isolated as completely as feasible from quarters occu- 

 pied by other pigs sick of hog cholera. All reasonable 

 precautions shall be taken to prevent infection of these 

 pigs from sources other than by inoculation. Such pre- 

 cautions shall include a thorough cleaning and disinfec- 

 tion of the pen in which the pigs are held during the 

 test, and a disinfection of these animals after they are 

 placed in a holding pen. The disinfection of these pens 

 and the test pigs shall be accomplished by such methods 

 as shall be approved by the chief of bureau. 



SECTION 4. Paragraph 1. Simultaneous virus shall be 

 collected in batches of not to exceed 20,000 cubic centi- 

 meters each and each batch shall be mixed thoroughly in 

 a single container. 



Paragraph 2. After mixing, but before phenoliza- 



