280 HOG CHOLERA 



from abrasions, and not less than 3 nor more than 12 

 months old, shall be furnished by the establishment for 

 the inoculation with the virus-test sample. 



Paragraph 7. All animals used for the testing of 

 simultaneous virus shall be inoculated only under the 

 supervision of a veterinary inspector, and shall be 

 marked as provided in paragraphs 2, 3, 4, and 5, section 

 6 of Kegulation 17. 



Paragraph 8. Each of the calves selected for testing 

 the purity of simultaneous virus shall be inoculated by 

 injecting 5 cubic centimeters of the virus-test sample 

 into either the auricular or jugular vein within 24 hours 

 after the virus is collected. 



Paragraph 9. Calves inoculated for the purpose of 

 determining the purity of simultaneous virus as pro- 

 vided in the preceding paragraph shall be held under 

 the observation of a veterinary inspector for a period of 

 at least 7 days. Should foot-and-mouth disease appear 

 in the United States the said calves shall be held under 

 the observation of a veterinary inspector for 10 days 

 or longer, in the discretion of the inspector in charge. 



Paragraph 10. Simultaneous virus which has been 

 either heated, as provided in paragraph 6, section 3 of 

 this regulation, or subjected to the test prescribed in 

 paragraph 8, section 4 of this regulation, may be re- 

 leased for marketing, provided the animals treated with 

 the virus remain well and develop no symptoms of any 

 infectious, contagious, or communicable disease. 



Paragraph 11. Simultaneous virus found to be worth- 

 less or contaminated shall be destroyed as provided in 

 section 11, Regulation 8, under the supervision of a 

 bureau employee. 



SECTION 5. Paragraph 1. Each immediate or true 

 container of simultaneous virus which has been tested 

 and found not to be worthless or contaminated shall bear 

 a^stamp or mark approved by the department. Such 



