282 HOG CHOLERA 



REGULATION 19. ANTI-HOG-CHOLERA SERUM 



SECTION 1. All operations incident to the production 

 of anti-hog-cholera serum shall be conducted under the 

 supervision of a bureau employee. Each licensed estab- 

 lishment shall notify the inspector in charge, or an as- 

 sistant, a reasonable time in advance when any opera- 

 tions are to be conducted. 



SECTION 2. Paragraph 1. Anti-hog-cholera serum 

 shall be derived only from' hyperimmune hogs which 

 have been immune to hog cholera for at least 60 days 

 prior to hyperimmunization. 



Paragraph 2. Anti-hog-cholera serum shall be derived 

 only from hyperimmune hogs which have been subjected 

 to not more than 4 successive bleedings after each hyper- 

 immunization. The first bleeding shall take place not 

 earlier than 10 days after hyperimmunization, subse- 

 quent bleedings shall not take place more frequently 

 than once in 7 days, and the last bleeding shall be made 

 on a date not later than 38 days after hyperimmuniza- 

 tion. 



Paragraph 3. Hogs which are used to produce anti- 

 hog-cholera serum shall be healthy at the time of hyper- 

 immunization, this fact to be determined by a careful 

 examination made by a veterinary inspector prior to 

 hyperimmunization. The temperature and weight of 

 each animal shall be accurately obtained and recorded 

 by the establishment before hyperimmunization. 



Paragraph 4. All hogs which are used to produce 

 anti-hog-cholera serum shall receive a single intravenous 

 injection of at least 5 cubic centimeters of hog-cholera 

 virus for each pound of the animal's weight. 



Paragraph 5. Temperature of all hogs used to pro- 

 duce anti-hog-cholera serum shall be accurately taken 

 and recorded by licensed establishments, either on the 

 afternoon before or on the day of bleeding, and at such 



