284 HOG CHOLEKA 



rived from each hyperimmune hog may be appropriately 

 mixed with serum from other hyperimmune hogs imme- 

 diately after collection, provided the final batch or mix- 

 ture is prepared as prescribed in the following para- 

 graphs of this section. 



Paragraph 10. Heating of anti-hog-cholera serum 

 shall be conducted under the supervision of a bureau 

 employee and in a manner in which the product and the 

 entire container thereof will be subjected to a tempera- 

 ture ranging from 59 to 60 C. for 30 minutes. 



Paragraph 11. Anti-hog-cholera serum which has 

 been heated as provided in the preceding paragraph 

 shall not be handled thereafter in a manner which will 

 expose the product to contamination. Final mixtures or 

 batches of anti-hog-cholera serum shall contain relative 

 proportions of the several bleedings. Single bleedings 

 from each hog shall not be divided or become a part of 

 two or more batches unless the serum is subjected to 

 heat as described in paragraph 10 of this section. 



Paragraph 12. Anti-hog-cholera serum which is to 

 constitute a batch or portion thereof may be strained 

 into a single container, after which the amount should 

 be accurately determined. 



Paragraph 13. Anti-hog-cholera serum shall have 

 added thereto a sufficient quantity of a 5 per cent solu- 

 tion of phenol to make the serum contain one-half of 1 

 per cent of phenol by volume. 



Paragraph 14. Phenolization of anti-hog-cholera se- 

 rum must be accomplished with accuracy, and in a man- 

 ner which will prevent the occurrence of undesirable 

 changes in the product. 



Paragraph 15. All records shall indicate clearly the 

 particular hog or group of hogs from which each batch 

 of serum or portion thereof is derived. The amount 

 prepared for phenolization and the total amount after 

 phenolization shall be separately recorded. 



