APPENDIX 285 



SECTION 3. Paragraph 1. Anti-hog-cholera serum 

 prior to testing shall be collected in batches of not more 

 than 100,000 cubic centimeters each, which shall be 

 thoroughly mixed in a single container. After mixing 

 and phenolizing, a representative sample consisting of 

 at least 375 cubic centimeters collected in three contain- 

 ers of not less than 25 centimeters, each to be known as 

 the "Serum-test sample," shall be taken and marked 

 with identifying marks by a bureau employee. The se- 

 rum, together with the test sample, shall be placed under 

 bureau lock, as provided under (b) section 1, Regula- 

 tion 7, and so held until such time as the tests required 

 by these regulations have been completed, and have 

 shown that the serum is not worthless, contaminated, 

 dangerous, or harmful. 



Paragraph 2. If the serum is released, one of the 

 three containers of the test sample thereof shall be. held 

 under bureau lock for at least six months after the latest 

 return date shown on the trade labels affixed to the im- 

 mediate or true containers of the serum of which the test 

 sample is a part. 



SECTION 4. Paragraph 1. All anti-hog-cholera serum 

 shall be tested for purity and potency by licensed estab- 

 lishments as prescribed by these regulations. 



Paragraph 2. For use in testing each batch of 100,- 

 000 cubic centimeters of anti-hog-cholera serum of less, 

 7 healthy pigs, susceptible to hog cholera and weighing 

 not less than 45 pounds and not more than 90 pounds 

 each, shall be furnished by the establishment. 



Paragraph 3. Each of the seven pigs furnished for 

 the test shall be injected with 2 cubic centimeters of 

 hog-cholera virus; of these pigs 5 shall receive 20 cubic 

 centimeters of the serum which is to be tested. Two of 

 the pigs shall receive no serum and shall serve as con- 

 trols. The virus and serum injections shall be made 

 simultaneously, the virus being injected into the left and 



