290 HOG CHOLEEA 



it is either concentrated, refined and tested in a manner 

 approved by the chief of bureau, or mixed with other 

 serum and tested as provided in section 7 of this regu- 

 lation. 



SECTION 7. Paragraph 1. When it is desired to mar- 

 ket anti-hog-cholera serum without concentration and re- 

 finement which has been tested with the results indi- 

 cated in paragraph 2, section 6, of this regulation, it 

 shall be mixed with other anti-hog-cholera serum with 

 the view of increasing its potency and the final mixture 

 shall consist of not less than 50 per cent nor more than 

 60 per cent of the serum of doubtful potency. 



Paragraph 2. Anti-hog-cholera serum which has been 

 mixed as provided in the preceding paragraph shall be 

 tested as outlined in sections 4 and 5 of this regulation, 

 with the following exceptions: 



(a) Eleven pigs in lieu of the 7 shall be used, 3 of 

 which shall receive virus only and shall serve as controls. 



(&) Unless two of the control pigs exhibit visible 

 symptoms of hog cholera subsequent to the fourth day 

 of the test period, but within 7 days after the test is 

 inaugurated and are sick of this disease to a degree suffi- 

 cient to result in death within 15 days after the time 

 of inoculation, "No test" will be declared. 



Paragraph 3. A second test conducted in the same 

 manner as before may be made of serum mixed as pro- 

 vided in paragraph 1 of this section should the results of 

 the test of the mixture be declared "Unsatisfactory" as 

 to potency. 



SECTION 8. Paragraph 1. Should abscesses develop 

 at the sites of the serum inoculations in any of the pigs 

 used for testing serum as provided in this regulation, 

 the following rules shall apply : 



(a) Judgment of the results of tests made on pigs to 

 determine the potency of anti-hog-cholera serum will be 



