APPENDIX 291 



rendered irrespective of those conditions found which 

 are regarded as an index to the purity of the product. 



(6) Should the results of a test of an ti -hog-cholera 

 serum be declared "Satisfactory for purity," and it is 

 found necessary to subject the batch of serum to a re- 

 test to determine its potency, judgment concerning 

 the purity of the product shall be based upon the 

 first test unless evidence is found subsequent to such 

 test which indicates that the serum is in fact contami- 

 nated. 



(c) Should the results of a test of anti-hog-cholera 

 serum be declared ' ' Satisfactory for potency ' ' but ' ' Un- 

 satisfactory for purity" the product may again be tested 

 for purity upon the same number of pigs as provided 

 under (a), paragraph 2, section 7, of this regulation 

 provided each pig receives a single injection, in the 

 axillary space, of at least 25 cubic centimeters of the 

 product to be tested. Immune pigs may be used for this 

 test if desired, and they shall be held under the super- 

 vision of a bureau employee for at least 15 days. 



Paragraph 2. Anti-hog-cholera serum may be released 

 for marketing as prescribed in paragraph 1, section 6, 

 of this regulation, after having been tested, as provided 

 by sections 7 and 8 and found satisfactory for purity 

 and potency. 



SECTION 9. Paragraph 1. Anti-hog-cholera serum 

 which has been tested twice with unsatisfactory results 

 as to purity as provided in 3 of rule C of this regulation 

 but satisfactory as to potency may again be tested with 

 the view of ascertaining whether it is in fact contami- 

 nated with pus-producing organisms, by treating 50 

 hogs on the premises of the manufacturing establish- 

 ment. The serum shall be administered under the super- 

 vision of a bureau employee, and each hog treated shall 

 receive a single injection, in the axillary space, of not 



