58 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



There were several small ulcers on the surface of the body; the right fore foot 

 swollen. From the spleen cultures were made on agar, potato, and in beef infu- 

 sion. All remained sterile. The other guinea pig recovered. The inguinal gland 

 had been slightly enlarged at one time. 



Guinea pig No. 4, inoculated with nasal mucus April 13, showed only slight en- 

 largement of axillary and inguinal lymphatics May 5. Four days later, the right 

 fore leg was badly swollen, and an ulcer had formed : 11 the top of the nose. It was 

 killed with chloroform May 12, twenty-nine days after inoculation. At this time 

 the swelling of fore leg had subsided, the place of inoculation had healed, but the 

 neighboring inguinal gland was one-half inch in diameter. Spleen slightly enlarged, 

 very pale. Liver dark. In neither are nodules discernible. Cultures were made 

 from spleen and the enlarged gland. The hair was carefully clipped away and the 

 skin blackened with a red-hot spatula in the line of the proposed incision. A flamed 

 knife was then thrust into the gland, which was found converted into a soft, white, 

 cheesy mass. The agar cultures from spleen remain sterile. Those from inguinal 

 gland contain on the third day colonies of glanders bacilli. On the potato inocu- 

 lated from the gland about twenty translucent, serum-like colonies appear from 1 

 to 2 millimeters in diameter. Examination shows them to be made up of glanders 

 bacilli. 



Bacilli from this culture, suspended in sterile beef infusion, were injected sub- 

 cutaneoiasly into a guinea, pig on the left side of abdomen May 15. Five days later 

 the testicles began to enlarge, arid within three weeks they were both 1-J- inches in 

 diameter. The animal was killed June 4. The spleen was slightly enlarged, but 

 without nodules. At the place of inoculation a fluctuating mass under the skin, 

 about one-half inch in diameter, filled with a soft, cheesy, yellowish-white pus. 

 On both testicles were ragged, blackish depressions, as if the skin either had broken 

 or was about to do so. The contents of both were entirely converted in yellowish- 

 white, cheesy pus. From one of them cultures were made", as indicated above. On 

 the third day typical colonies of glanders bacilli had appeared. 



III. April 6. two guinea pigs were inoculated subcutaneously with scrapings from 

 the base of ulcers on the nasal septum of a horse killed for glanders. Up to May 

 10 the place of inoculation had 'healed in both cases. In one, an inguinal gland and 

 the testicles were enlarging. May 28, this one was killed, as it was growing gradu- 

 ally worse. At the autopsy the left inguinal gland was about one-half inch in di- 

 ameter, the contents easily expressed as a thick creamy pus. The inguinal gland 

 of the other side about half as large. The left testicle has a broad scar with black- 

 ened base, evidently a rupture through "which the contents had been discharged, 

 which accounts for the shrunken size of the gland. The other testicle consists of 

 two enormous abscesses, apparently not communicating with each other (testicle 

 and epididymis), containing liquid pus. Between the nose and the right eye an 

 ulcer with a blackish dried-up base and irregular margins. Two days later a potato 

 culture, made from the spleen, contains a moderate number of isolated colonies 

 characteristic of glanders. An agar culture did not grow. From one testicle two 

 agar cultures contain numerous colonies. From the enlarged inguinal gland both 

 an agar and a potato culture developed numerous colonies. These were character- 

 istic, both rnacroscopically and microscopically, of glanders. 



From the potato culture of the inguinal gland, when ten days old, two mice were 

 inoculated by injecting subcutaneously a few drops of a suspension of bacilli in 

 sterile bouillon. Both remained well. " The second guinea pig, inoculated from the 

 same horse, did not take the disease. 



IV. April 27, a young female guinea pig and a rabbit were inoculated with pus 

 from farcy bud^glanders of the skin). The rabbit remained well; the inoculation 

 wound was healed at the end of two weeks. In the guinea pig the wound had also 

 healed, but the inguinal gland was beginning to enlarge. May 16 the gland was 

 much larger, and the labium on the same side was beginning to swell up. May 20, 

 a swelling about the size of a walnut appeared under the skin above the hip of op- 

 posite side. This broke later on. Found dead May 24. Besides the lesions already 

 mentioned both fore feet and the right hind foot" were swollen. The contents of 

 the enlarged inguinal gland were puriform. In the labium a small abscess. Cult- 

 ures were made on various media from th6 spleen, lymph gland, swollen labium, 

 and blood. Those from the spleen and gland were pure cultures of glanders bacilli; 

 those from labium impure. 



From the s|leen and inguinal gland of this guinea pig two young male guinea 

 pigs were inoculated May 24, each receiving a minute portion from both sources. 

 June 7 one was killed. Both fore feet are swollen over the carpal and metacarpal 

 joints; the right has a depressed place concealed by a scab. The left hind linab is 

 also swollen over the tibio-tarsal joint. The place of inoculation is covered by a 

 scab. The inguinal gland and testicles are not swollen. The internal organs are 



