KEPOET OF THE BUKEAU OF ANIMAL INDUSTRY. 59 



not visibly altered. Cultures were only made from spleen pulp on agar, which 

 remained sterile. 



The second guinea pig recovered. 



V. June 4, from the nasal discharge of a horse affected with glanders, two guinea 

 pigs, two young rabbits, and a young dog were inoculated, all in the same manner 

 under the skin, as already indicated. One of the guinea pigs died July 9. It had 

 a large ulcer with black dried-up base behind the left nostril, three on abdomen, 

 and one on the right flank three-fourths inch in diameter. There was also an ulcer 

 011 the left heel and right fore limb. The right heel was considerably swollen. The 

 left testicle was also quite large. The spleen was free from nodules. Cultures on 

 agar and potato were made from the spleen only. These were found subsequently 

 to be impure. 



The second guinea pig had large swellings in the groin, and was unable to use its 

 hind limbs for a time, but it finally recovered. The rabbits and dog were not af- 

 fected. At the autopsy of the horse, from which these had been inoculated, the 

 left nasal passage and adjacent sinuses had the mucous membrane partly thickened 

 and eroded. There were also nodules in the lung tissue. 



VI. July 27, three guinea pigs were inoculated subcutaneously as before with 

 nasal discharge from a horse affected with glanders. One of these, a small male, 

 died August 11. On the right side of abdomen an ulcer about three-eighths of an inch 

 in diameter, with a cup-shaped, dark, dry crust covering it. When removed, a soft, 

 cheesy mass, involving subjacent muscles, is exposed to view; serous surface not 

 involved. No enlargement of gland. Putrefaction had set in and no cultures were 

 therefore made. 



At this time a peculiar disease was carrying off healthy guinea pigs quite rapidly, 

 which was traced to the dry food given them. It is probable that this guinea pig 

 also died of this disease, although evidently infected with glanders. The second 

 guinea pig died during my absence and no examination wa$ made. The third, 

 however, developed glanders in a marked degree and was killed August 23, twenty- 

 seven days after inoculation. On the right side of abdomen, near median line, an 

 indurated mass covered by a black crust. The abdominal muscles and the peri- 

 toneal covering are intact, but between them and the crust is a cheesy mass. Simi- 

 lar ulcers exist on right flank and on right thigh externally. The right fore limb 

 and both hind limbs are swollen, especially the right hind limb. This is three-quar- 

 ters of an inch thick, bluish, ulcerated between first and second toe. Internal 

 organs seem unchanged, although the spleen contained glanders bacilli. On each 

 of three potato cultures from this organ from 15 to 25 typical colonies appeared 

 within three days. 



VII. September 21, two guinea pigs were inoculated with nasal mucus from a 

 horse suspected to be affected with glanders. One died October 12. There were 

 two ulcers on the abdomen about one-half inch across, the floor of which was 

 formed by the muscular wall of the abdomen. The inguinal gland of the same 

 side as large as a walnut and containing a cream-colored, liquid pus. Spleen, not 

 enlarged, contains a large number of minute whitish dots. 



In all cultures on potato, from both spleen and pus of inguinal gland, the typical 

 pale yellowish colonies of glanders subsequently appeared. The second guinea pig 

 was killedwith chloroform October 16. At the place of inoculation a small ulcer 

 had formed and the nearest inguinal lymph gland was equal in size to a small mar- 

 ble. The left testicle was enlarged and contained a pus cavity. The spleen small. 

 From the pus of the testicle an agar culture was prepared; a potato culture from 

 the spleen. Only the former developed colonies of glanders bacilli. 



VIII. September 22, two guinea pigs, inoculated with nasal mucus from a horse 

 probably affected with glanders. One died September 31, very much emaciated. 

 Over lowest ribs on the left side a cheesy nodule, about one-half inch in diameter, 

 representing the place of inoculation. The inguinal gland of same side as large as 

 a small bean. On the same side the subcutaneous tissue is infiltrated with a trans- 

 lucent exudate, sufficient to make the surface of the muscles and under surface of 

 the skin glistening and slippery (malignant oedema?). In spleen about 15 tubercles, 

 grayish, 1 millimeter (one-twenty-fifth inch) across; on the various lobes of the liver 

 similar but smaller tubercles; on lungs grayish subpleural nodules, irregular in out- 

 line, embedded in areas of a dark red hepatization. 



Two potato cultures were made from spleen tissue and within three days twenty 

 to thirty drop-like colonies, resembling a pale-yellow serum, so characteristic of 

 glanders bacilli, made their appearance. 



The second guinea pig was chloroformed October 15. In the left groin the lymph 

 gland is converted into an abscess, about the size of a marble, containing a soft, 

 cheesy, cream-colored pus. The right testicle, enlarged, contains an abscess; a few 

 nodules in spleen. A potato culture from the latter organ failed to develop. 



IX. November 29, from a horse killed and found glandered, a guinea pig was in- 



