BEPOET OF THE BUKEATT OF ANIMAL INDUSTRY. 65 



1 per cent, solution of carbolic acid in less than ten minutes, and by 

 a momentary contact with water near the boiling point, the disinfec- 

 tion was certainly all that could be desired. This method was re- 

 garded as less open to criticism than the insertion of bits of tissue 

 under the skin. We still stand in need of a syringe which can be 

 disinfected without much trouble, as the above method is extremely 

 tedious. The syringes devised by Koch are both unsatisfactory. 

 The joints formed by the glass barrel and the metal cap in the syringe 

 in which the propelling force is air were found to leak in five out of 

 six samples. From the fluid injected into rabbits either plate or roll 

 cultures were made in order to get an idea of the approximate num- 

 ber and the kind of organisms present. In every case the portion of 

 lung tissue from which the inoculations were made was transferred 

 to sterilized bottles and protected from accidental contamination as 

 carefully as possible, unless otherwise indicated, the methods just 

 given were employed throughout the investigation. 



November 16. Pig No. 1 just died and brought to Experimental 

 Station. No skin lesions; heart and lungs normal with exception of 

 a few collapsed lobules in principal lobe of one lung. * In abdomen 

 omentum injected so as to appear bright red. Spleen enlarged, soft, 

 dark. Some lymphatics have the cortex hemorrhagic. Stomach and 

 small intestines normal. In caecum near valve several large super- 

 ficial yellowish ulcers anfl a number of smaller ones, an eighth of an 

 inch in diameter. Two bits of spleen were cut out and dropped into 

 a tube of gelatine, and one of beef infusion. The gelatine culture 

 remains sterile. The liquid culture contains the motile hog cholera 

 bacteria and a large butyric bacillus growing only in the bottom of 

 the liquid. 



No. 2 died last night. Buzzards had eaten into the thorax and pene- 

 trated the coats of the stomach. Only the small ventral lobe of right 

 lung diseased. Bright red, mottled with pale yellow dots. (See 

 Plate V, fig. 2.) The smallest bronchi occluded bv cylindrical plugs. 

 Alveoli likewise occluded. The plugs consist chiefly of cells and 

 are so dry and firm that they may be removed as small ramifications 

 or branchings when the lung tissue is torn away. Bronchial glands 

 enlarged, hemorrhagic. Spleen enormously enlarged, liver in ad- 

 vanced stage of cirrhosis. Glands at portal fissure chronically en- 

 larged. In csecum four superficial ulcers one-half inch across, slough 

 stained yellow. In upper colon four similar to these and a large 



* In order to understand the description of the lung lesions, the following brief 

 outline of the anatomy of the lung and of the terms used may be of service: 



The right lung is made up of four lobes; the left has only three. (In text-books 

 on anatomy the left lung is considered as being made up of only two.) 



In both there is a large principal lobe resting upon the diaphragm and against the 

 adjacent thoracic wall. This lobe forms the major part of each lung. The remain- 

 der, occupying the anterior (or cephalic) portion or the cavity, is made up of two 

 small lobes, one extending ventrally (or downward in the standing position of the 

 animal) and in the expanded state covering the heart laterally, the other extending 

 towards the head and overlapping the base of the heart. These small lobes may be 

 denominated the ventral and cephalic lobes, respectively. The right cephalic lobe 

 is longer and more distinct from the ventral lobe than the corresponding left cephalic 

 Wedged in between the two principal lobes and resting on the diaphragm is a small 

 lobe, pyramidal, belonging to the right lung (azygos lobe). This lobe rests on the 1 

 left against the mediastinal membrane, and on the right it is separated from the right 

 principal lobe by a fold of the pleura passing from the ventral abdominal wall to 

 inclose the inferior vena cava. This small lobe is almost completely shut off, there- 

 fore, from the other lobes by folds of the pleura. 



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