66 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



number of small ones about one-eighth inch across with yellowish 

 slough. 



A moderate number of bacteria found in cover-glass preparations of 

 spleen. A gelatine tube culture contained, after three days, about sev- 

 enty-five to one hundred colonies of the motile hog cholera bacteria in 

 each needle track. A liquid culture inoculated simply with the 

 platinum wire thrust into the spleen pulp contained hog cholera bac- 

 teria only. A rabbit was inoculated from lung tissue as above de- 

 scribed; about one-fourth of a cubic centimeter of the suspension 

 injected. Dead on the seventh day. Slight fibrinous exudate on 

 coils of intestines. Spleen very large, soft, dark. Beginning coagu- 

 lation-necrosis in liver. Bacteria very numerous in spleen; both 

 gelatine and liquid cultures contain only the motile hog cholera bac- 

 teria. The plate from lung tissue with which the rabbit was inocu- 

 lated contains a very large number of identical colonies, made up of 

 motile hog cholera bacteria. 



November 17. No. 3, male, died yesterday. Buzzards have con- 

 sumed pectoral muscles and pierced into thorax and abdomen. 

 Ecchymoses on costal pleura and entire epicardium, a few under 

 pulmonary pleura. Left lung hypostatic, slight amount of whitish 

 foam in trachea. Lung tissue otherwise entirely normal, neither 

 hepatization nor collapse anywhere to be seen. * Bronchial glands 

 and those along posterior aorta with hemorrhagic cortex. Ecchy- 

 moses in the subcutaneous fatty tissue over entire ventral aspect of 

 body, about one-eighth inch across, beneath peritoneum of abdomi- 

 nal muscles and of the entire length of small intestine, from the size 

 of a pin's head to one-eighth inch. Congestion in patches in large 

 intestine, no ulceration. Stomach and intestines contain a yellow 

 liquid resembling the yolk of eggs. Spleen but slightly enlarged. 

 Ecchymoses under serosa of liver. Cirrhosis moderate. A bit of 

 spleen tissue dropped into a tube of beef infusion gave rise to a 

 culture of hog cholera bacteria. In each needle track of a gelatine 

 culture countless colonies of the motile bacteria appear. 



November 18. No. 4, medium-sized male, died this morning. Su- 

 perficial inguinal glands enlarged, cortex slightly hemorrhagic. 

 Some fibrils on coils of intestine. Petecchise under serosa of small 

 intestine; extensive hemorrhage between mucous and muscular coat 

 of stomach along fundus, forming a clot about one-half inch thick. 

 Spleen very large, friable, blackish, extends beyond median line into 

 right side. Petecchise on liver, which is considerably cirrhosed; mu- 

 cosa of stomach along fundus blackish; closely set petecchise under 

 mucosa of small intestine; contents liquid, blood-stained. A dark 

 hemorrhagic patch near valve in large intestine. Colon studded 

 with petecchise, no ulceration. Small number of petecchise on sur- 

 face and throughout cortex of kidneys. No bacteria seen on a cover- 

 glass preparation of spleen tissue. Beef infusion inoculated with a 

 wire thrust into spleen remains sterile. A bit of spleen dropped into 

 a tube of gelatine gives rise to about a dozen colonies of hog cholera 

 bacteria. 



November^ 18. No. 5, male, 75 pounds, three to four months old, 

 died last night. Inguinal glands enlarged, pale. Left cephalic, 

 ventral, and about one-third of principal, right cephalic and ventral 

 lobes of lungs solidified, bright red, mottled with minute yellowish 

 dots, i. e., same as No. 2. Costal pleura covered with a very thin 

 whitish exudate. Solidified portion of principal lobe adherent. On 

 epicardium a very delicate papery deposit. Spleen much congested. 



