90 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 







held in the position normally occupied in the body, as if the agencies producing the 

 lesion had settled into the most dependent portions and there began their patho- 

 logical action first. The portions affected were thus the small ventral lobes resting 

 on the pericardium, these being lowest in the natural position of the animal, and 

 portions of the cephalic and principal lobes. Those portions resting against the 

 vertebras were still normal. 



The color of the hepatized regions was grayish red to reddish yellow, sprinkled 

 with closely set yellowish white spots from one-half millimeter to 5 millimeters 

 in diameter and slightly prominent. On section the same appearance presents 

 throughout the diseased lung tissue. The recently affected lobules are either uni- 

 formly and deeply reddened or sprinkled with a large number of very minute whitish 

 points. Odor slightly putrefactive. 



The yellowish- white masses were firm and cut with a smooth surface. Micro- 

 scopic examination showed them to be made up of broken-dow T n cells, among 

 which were immense numbers of various kinds of bacteria. These lesions were 

 evidently produced by the same cause that we had studied in western outbreaks. 

 There was but one way to speedily isolate the specific microbe from the mass of 

 other microbes which were living in the dead cell masses. Three mice were in- 

 oculated by placing beneath the skin of the back bits of lung tissue from the re- 

 cently diseased areas. A rabbit was inoculated in the thigh in the same way by 

 making an incision through the skin, which had been disinfected with .1 per cent, 

 mercuric chloride, introducing a bit of lung tissue and drawing together the in- 

 cision with a stitch. 



The rabbit died within 24 hours. Very slight purulent envelope about bit of 

 tissue. No other reaction locally. Nearest lymphatic hemorrhagic. In it, as well 

 as in blood from the heart, spleen, and liver, immense numbers of the polar- 

 stained bacteria. Cultures in gelatine and liquid from, blood pure. Two mice died 

 on the third day. Bacteria present in small numbers. The third died on the fourth 

 day. Immense numbers of the same bacteria in blood from heart. Pure cultures 

 from the latter organ were obtained from each mouse. 



Pig No. 405 (bit of lung tissue). 



rabbit, Feb. IS 3 mice, Feb. 18 



died Feb. 19 died Feb. 21, 22 



(swine plague bacteria only in cultures), (cultures from blood contain only swine plague bacteria). 



One of the most pronounced cases of this disease died February 21, and was ex- 

 amined on the following day. 



No. 407. Pig of medium "size, white; skin of abdomen, chest, neck, and back 

 deeply reddened. Fat abundant, slightly reddened along the linea alba. Superfi- 

 cial inguinals slightly enlarged; spleen dotted with elevated blood-red points. Liver 

 very dark. Stomach and duodenum normal, the latter bile-stained. In ileum 

 Peyer's patches are visible as groups of small, dark dots; no swelling. Mucosa of 

 caecum and upper colon of a dirty, blackish color, probably pigmented. A few 

 haematomata beneath mucosa. Besides the diffuse pigmentation the mucosa is 

 sprinkled with isolated or confluent masses, about one-eighth to one-fourth inch in 

 diameter, of a dirty grayish-yellow color, loosely adherent to the membrane. When 

 pulled away a slightly depressed surface is exposed. Much of this mass can be 

 easily removed by simply moving the scalpel over it. There are several ulcers in 

 the caecum with decided loss of substance. The patch of mucous glands at the base 

 of the valve is also converted into an ulcerated mass. Lymphatic glands in abdo- 

 men slightly swollen and reddened. Kidneys deeply reddened to tips of papillae. 



On opening the thorax the lungs did not collapse, and a rather disagreeable odor 

 was perceived. As in No. 405, the ventral and cephalic lobes of both lungs were 

 consolidated. The hepatized regions were very hard to the touch, bright red, with 

 yellowish points sprinkled in regularly. (See Plates I, II, III, fig. 1.) The right lung 

 was adherent to chest wall along the hepatized portion. A whitish, spongy membrane 

 was interposed, about 3 millimeters to 5 millimeters thick, inclosing a small quantity 

 of turbid liquid. On removing the lung the membrane remained adherent to pul- 

 monary pleura and was removed with difficulty. A portion of the diaphragm was 

 also firmly attached. The left lung adhered firmly to the chest wall in two places 

 where it was consolidated. The costal pleura was deeply reddened, owing to the 

 injection of a close net-work of minute vessels. Trachea and bronchi filled with 

 whitish foam. 



On section, the consolidated region is sharply but irregularly marked off from, 

 the normal tissue, very consistent and slightly elevated. The color varies from a 



