94 EEPOET OF THE BUREAU OF ANIMAL INDUSTRY. 



median (azygos) lobe is solidified along the border in several places. Right heart 

 filled with a large thrombus, firmly adherent to papillary muscles. Left heart con- 

 tains a small dark clot. Large vessels near heart and in lungs distended with white 

 thrombi. 



Bacterial investigations negative. One rabbit inoculated with bit of lung tissue 

 in thigh remained Avell. Two mice inoculated with lung tissue died on the follow- 

 ing day. Cultures from blood remained sterile. All but one culture from spleen 

 and liver remained sterile. This contained a bacillus growing in the bottom of the 

 liquid (butyricus ?). 



In order to test the communicable character of this lung disease, two healthy 

 pigs (Nos. 359, 360) were taken February 28 to the farm and penned with Nos. 

 408, 409, 410. At this time two of these three were already diseased, and No. 407 had 

 already died in the same yard. On March 16, No. 408, having meanwhile suc- 

 cumbed to the disease, the remaining four were taken back to a clean pen on the 

 Experimental Station. No. 409 died March 20, as already stated, and No. 359 was 

 found dead March 24. It had been so weak as to be scarcely able to stand, al- 

 though apparently free from cough. No skin lesions; superficial inguinal glands 

 enlarged, pale, medullary portion dotted with blood-red points. A few slender 

 fibrinous strings stretched across the coils of intestine. Stomach contracted, contain- 

 ing about 50 cubic centimeters of a turbid yellow liquid. The wails covered with tena- 

 cious bile-stained mucus. Gall bladder contracted; contains an ascaris; one in cystic 

 duct. Mucosa of large intestine dark colored. In caecum four or five large ulcers, 

 one surrounding base of valve. In another, about ofle-half inch across, the necrotic 

 center projected like a button. In colon a large mkinber of small ragged patches 

 of a thin deposit which seems to be dead epithelium. Thorax. Lungs do not col- 

 lapse when thorax is opened. The major portion of both, excepting dorsal region, 

 closely adherent to chest wall. The attachment being severed without difficulty, 

 both pleural surfaces are found covered with a thin grayish deposit. Small quan- 

 tity of turbid liquid present in pleural cavity; diaphragm entireJy but loosely adhe- 

 rent to the pulmonary pleura. Portion of pericardium also adherent to pleura on 

 the right side. 



When the lungs were removed from thorax, all but the dorsal region found solidi- 

 fied. Even in this there were scattered hepatized lobules. The lung tissue varies in 

 color from a pale to a bright red and resembles the diseased lungs already described. 

 The smaller bronchi of the affected lobes were, as a rule, occluded with more or less 

 consistent whitish plugs. Bronchial glands enlarged, cortex bright red. 

 ^ Heart but slightly distended. Right ventricle filled with a firm washed clot ; 

 right auricle with a dark, soft clot. Left ventricle contains a small, dark clot ; left 

 auricle the same. This latter is dotted with closely-set, bright-red petecchiae, as if 

 sprinkled with blood. Great vessels filled with dark clots. 



Microscopic examination reveals a large number of swine plague bacteria in 

 pleural exudate, immense numbers in the lung tissue. The whitish plugs in the 

 smal! air tubes contain a moderate number mingled with other forms. Sections of 

 the same lung tissue showed immense numbers of swine plague bacteria among 

 the alveolar exudate. Bacilli were also present, but in small number. The follow- 

 ing cultures were made: Of two beef infusion cultures from the pleural exudate 

 on<3 contained the swine plague bacteria only, the other in addition a streptococcus. 

 With the pure culture two rabbits were inoculated by injecting one-fifth cubic centi- 

 meter beneath the skin of the thigh. One of them (small white) was dead next morn- 

 ing. A few ecchymoses in the subcutis at point of inoculation. The internal organs 

 appeared unchanged. Both spleen and liver contained immense numbers of the in- 

 jected bacteria pure cultures of which were obtained from blood of heart and liver. 

 The second rabbit was found dead on the third day. There was locally an extensive 

 infiltration of the subcutis with superficial degeneration of thigh and contiguous 

 abdominal muscles. Slight peritonitis. Hemorrhage into caudal lobe of right 

 lung. Very few bacteria in spleen, liver, and heart's blood. A tube culture" in 

 gelatine shows the colonies in a few days. 



Subsequent inoculations were made from cultures derived from the first rabbit. 

 Two rabbits were inoculated in the ear with a lancet dipped into the culture liquid. 

 Both died on the third and fourth day after inoculation. The swine plague bacteria 

 were found in the spleen of both. 



Of two gelatine cultures from the pleural exudate both are impure, one contain- 

 ing besides the swine plague bacteria also the streptococcus, the other a chroino- 

 genous bacillus, described as bacillus luteus in the Second Annual Report of the 

 Bureau (1885). Of three cultures from the spleen in beef infusion the bacillus 

 luteus is present as well as the swine plague bacteria. This chromogeiious organ- 

 ism had penetrated into the internal organs, the streptococcus being limited to the 

 pleural cavity. 



