EEPORT OF THE BUEEAU OF ANIMAL INDUSTRY. 97 



yellowish, projecting ulcer on base of valve. Four or five of the same character, 

 three-eighths to one-half inch across in csecum and upper colon. In this animal the 

 brain and spinal cord were laid bare. A few punctiform recent extravasations, 

 chiefly in the white matter of the cerebellum. 



With a bit of lung tissue two rabbits were inoculated one beneath skin of thigh, 

 another in the ear. Both were found dead within forty hours after inoculation; the 

 bacteria of swine plague present in spleen and liver in large numbers, and obtained 

 pure in cultures. 



From the spleen, pleural, and peritoneal exudate the hog cholera bacteria were 

 obtained pure. They were very scarce in the pleural exudate, however, as two out 

 of three liquid cultures remained sterile. Two mice inoculated from the spleen 

 culture were dead on the sixth day, both of hog cholera from the injected cultures. 



Pig No, 397 died April 14. 

 lung tissue 



No. 396 is another interesting case of lung disease complicated with hog cholera. 

 This animal had been fed February 22 with portions of the spleen and large intes- 

 tine of No. 407, which has been dwelt upon in the preceding pages. 



A few days after feeding it began to show signs of disease by growing weakness, 

 especially marked in the hind limbs, no desire for food, and a slight diarrhea. A 

 month after feeding, constipation set in, while the weakness of the hind limbs was 

 very marked, bordering on paralysis, and anorexia continued. In the seventh week 

 diarrhea again set in: the animal was unable to rise and died April 18, nearly two 

 months after feeding. It was found with severe and extensive lesions of the lungs 

 and large intestine. (See Plate IV.) 



Digestive tract. Stomach filled with food; normal. Large intestine, excepting 

 rectum, ulcerated. Ulcers surrounding valve and forming a confluent mass in 

 caecum. In upper colon there are masses of exudate from one-eighth to one-fourth 

 inch in diameter, at least 20 to a square inch (Plate IV, fig. 2); lower down fewer 

 in number. They are roundish, convex, brick-red masses which may be easily 

 lifted from a raw, slightlv depressed surface; every one is surrounded by an injected 

 border. The mucosa itself is of a bluish-green color. 



Lungs. Right ventral lobe firmly and closely adherent to chest wall by a con- 

 tinuous sheet of fibrous tissue. This lobe feels like a bag filled with hard round 

 bodies. On section it is found filled with whitish homogeneous masses resembling 

 hard cheese (Plate IV, fig. 1) embedded in bright-red, hepatized lung tissue. Left 

 cephalic lobe in the same stage. The remainder of the left lung contains groups of 

 lobules recently hepatized. Cavities of heart as in preceding cases. 



Two rabbits and two mice inoculated in the ears with lung tissue without any 

 result. 



Gelatine cultures from pleura remained -sterile. A culture from the spleen con- 

 tained several forms, one of which resembled the hog cholera bacillus. Alter 

 isolating this, two mice-were inoculated from a pure culture. Both died in seven 

 days with lesions characteristic of this disease. 



The hog cholera germs were therefore present in this animal. The swine plague 

 germ was not isolated, perhaps because the germs were too few. In such cases in- 

 oculation on the ears of rabbits seems to fail, since only a minimum quantity of lung 

 tissue comes in contact with the puncture. 



Whether the lung disease of this pig was contracted from the feeding must be 

 determined from additional cases. 



No. 392 died from swine plague, with a few doubtful hog cholera lesions and with 

 hog cholera bacteria in the spleen. The remainder of the exposed animals had no 

 swine plague lesions, but death was caused by acute hog cholera.* 



The history of this animal is instructive, as it was inoculated from a culture of 

 swine plague bacteria, which operation did not protect it from taking the disease 



* The next case of swine plague in this pen died June 29, over two months after 

 the death of No. 392. 



12057 A I 7 



