98 REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 



subsequently. January 25, 1887, injected into each thigh about 2 cubic centimeters 

 from a beef infusion peptone culture, one day old, of swine plague bacteria, obtained 

 from an Iowa outbreak. For several days after the hind limbs were stiff, appetite 

 poor, but the animal fully recovered. March 28, it "was transferred with some others 

 to a pen infected with swine plague and with hog cholera, as subsequent deaths 

 showed. Two weeks after the animal began to fail and died April 20. 



Skin along median line of abdomen deeply reddened. Subcutis over same region 

 and sides, and subperitoneal tissue dotted with numerous pale-red spots of extravasa- 

 tion. Liver cirrhosed. contracted, pale. Digestive tract. Mucosa along greater cur- 

 vature of stomach dotted with small extravasations. Near cardiac orifice the small 

 diverticulum contained from fifteen to twenty yellowish-white excrescences, round, 

 removed with difficulty, and leaving a raw, depressed surface (diphtheritic). Csecum 

 and upper two-thirds of colon pigmented. In the latter the summits of about seven 

 transverse ridges were covered with a very thin sheet of necrosed tissue. 



The lungs are the seat of recent and extensive disease. The ventral and cephalic 

 lobe and ventral region of principal lobe of both lungs, as well as the small azygos 

 lobe, are airless, of a deep red, mottled with pale-yellowish dots as in former cases; 

 these dots correspond to the alveoli filled with exudate. 



The disease is farthest advanced in the ventral and cephalic lobes. Here the lung 

 tissue is interspersed with hard, yellowish-white nodules, from the size of a pin's 

 head to that of a pea. Cover-glass preparations from the various diseased lobes show 

 numerous forms of bacteria, no one predominating. The remaining portions of the 

 lungs on the dorsal aspect are cedematous; a frothy liquid rapidly fills up the section. 

 Scattered over the entire surface of both lungs are subpleural ecchymoses. These 

 are characteristic of hog cholera in its most acute form. 



The inoculations are given in the appended table and may be very briefly sum- 

 marized. Plate cultures from lung tissue gave two forms, the bacteria of swine 

 plague and streptococci, mentioned in former cases. A rabbit inoculated from a 

 mixed culture of these two forms died in four days. The various cultures of internal 

 organs of this animal contained the swine plague bacteria only. Another rabbit in- 

 oculated from a pure culture of the streptococcus died in twenty days from an ab- 

 scess due to the inoculation. Two mice inoculated with lung tissue died. In one the 

 bacteria of swine plague were found in large numbers. Of three cultures of the 

 spleen of the pig only one became turbid. This contained hog cholera bacteria only, 

 as shown by the microscope and inoculation of mice. 



This pig had therefore the bacteria of two diseases, the lung lesions belonging to 

 one, the various hemorrhagic lesions, ecohymoses, etc. , belonging to the other. 



(swine plague) 



During March and April a few animals placed in the infected pen in which the 

 virus of both diseases had been scattered no longer took swine plague, but suc- 

 cumbed to hog cholera, as the following notes clearly indicate: 



Pig No. 390. placed in the infected pen March 22, with pigs which subsequently 

 died from lung disease as well as hog cholera. The animal died quite suddenly April 

 6. On examination the spleen was found very large, due to engorgement with 

 blood. A moderate number of hog cholera bacteria present. Fundus of stomach 

 considerably reddened. In the large intestine the mucosa is studded with irregular 

 masses simulating ulcers; most of them seem to be adherent fecal masses. A few, 

 when removed, leave a slightly depressed surface. Glands of meso-colon reddened; 

 lungs normal, with the exception of a few scattered lobules, whiqji are collapsed. No 

 swine plague lesions observable. 



The hog cholera bacteria were obtained in pure cultures from the spleen. Their 



