REPORT OF THE BUREAU OF ANIMAL INDUSTRY. 103 



ceived into the trachea each 5 cubic centimeters of a beef infusion 

 culture of swine plague bacteria about two days old. On the follow- 

 ing day both were well. On April 27 No. 389 was killed and found 

 healthy. No. 401, killed April 28, had likewise remained unaffected 

 by the inoculation. 



(c) At the same time, and with the same culture with which Nos. 

 383 and 385 were inoculated, two pigs (Nos. 352, 388) were inoculated 

 directly into the lungs through the chest wall. No. 352 received 2-J 

 cubic centimeters into the right lung with a hypodermic syringe, the 

 skin having been previously disinfected with a one-fifth per cent, 

 solution of mercuric chloride. No. 388 received 5 cubic centimeters 

 in the same way. Both animals lost the use of their limbs, especially 

 the posterior, within a day after the operation, remaining more or 

 less paralyzed until the animals were knled f April 26. In the mean 

 time they ate but little, while there were no symptoms directly refer- 

 able to lung disease. Shortly before they were killed they had al- 

 most gained control of their limbs. 



Autopsy of No. 352. Right lung firmly adherent to chest wall and 

 diaphragm by means of dense fibrous tissue. Left lung adherent in 

 a few places. On the lateral aspect of the left lung an oval mass as 

 large as a small fist was found inseparably attached to it. This mass 

 fluctuates, and when cut consists of a dense fibrous wall one-eighth 

 inch thick, its inner surface deep red. The contents of this sac 

 were of a putty-like consistency, greenish white, surrounded by a 

 turbid fluid containing small flakes. The mass consisted of degen- 

 erated pus corpuscles, in which were disseminated the swine plague 

 bacteria in moderate number. The lung tissue was merely com- 

 pressed by the tumor-like mass and not diseased. A gelatine tube 

 culture inoculated from the caseous contents of the abscess contained 

 an immense number of colonies of swine plague bacteria in each 

 needle track. The absence of other micro-organisms proves that the 

 abscess was the result of the presence of the injected bacteria. The 

 other organs were normal. Cultures from the spleen remained sterile. 



In No. 388 the pleuritic adhesions of the right lung are the same; 

 the left lung free. Along the lateral edge of the principal lobe of 

 the right lung are two tumors, one as large as a horse-chestnut, the 

 other, contiguous with it, about the size of a marble. The walls and 

 contents as in 352. A liquid and a gelatine tube culture from the 

 contents contain the swine plague bacteria only. In the latter the 

 colonies were innumerable in each needle track. The bacteria had 

 therefore not only lived for forty-one days, but had multiplied enor- 

 mously, causing the lesions described. 



On January 25 Nos. 391 and 392 received into the subcutaneous 

 tissue of the thighs 4 cubic centimeters of a beef infusion peptone 

 culture about twenty-four hours old. The swine plague bacteria 

 were obtained from an Iowa outbreak reported in 1886. 



No. 391 became lame soon after, probably from handling. Its ap- 

 petite, poor at first, was restored after a week. February 18 it was 

 killed, but no lesions were found, excepting circumscribed abscesses 

 on the thighs at the points of inoculation. 



No. 392 has been dwelt upon in the preceding pages. It did not 

 show any symptoms referable to the inoculation. When exposed in 

 an infected pen it died, being found with extensive disease of the 

 lungs. Hence the inoculation was in no sense protective. 



Pig No. 387, after a fast of more than twenty-four hours, was fed 

 March 8, with about 200 cubic centimeters of a beef infusion culture 



